Product Name:Mouse Lecithin retinol acyltransferase, Lrat ELISA KIT
Packing:96T
Catalog No.:ELI-42206m
Gene Name:Mouse Lrat
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Lrat
Alternative Name:Mouse Lecithin retinol acyltransferase, Lrat
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Lecithin retinol acyltransferase, Lrat ELISA KIT allows for the in vitro quantitative determination of Mouse Lrat concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Lecithin retinol acyltransferase, Lrat ELISA KIT has been pre-coated with an Mouse Lecithin retinol acyltransferase, Lrat antibody specific to Mouse Lrat .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lrat and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lrat , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lrat in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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