Mouse GRB2-related adaptor protein 2, GRAP2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GRAP2,GADS; GRAP-2; GRB2L; GRBLG; GRID; GRPL; GrbX; Grf40; Mona; P38; GRB-2-like protein; GRB2-related protein with insert domain; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule; adapter protein GRID; grf-40; grf40 adapter protein; growth factor receptor-binding protein; growth factor receptor-bound protein 2-related adaptor protein 2; hematopoietic cell-associated adapter protein GrpL; hematopoietic cell-associated adaptor protein GRPL; GRB2-related adaptor protein 2, GRB-2-related monocytic adapter protein,MONA,Monocytic adapter,GRB-2-like protein,GADS protein,
Search name
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse grb2-related adaptor protein 2,GRAP2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRAP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GRAP2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GRAP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GRAP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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