Mouse Appetite-regulating hormone, GHRL ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Appetite-regulating hormone,Protein M46,Motilin-related peptide,Growth hormone secretagogue,Growth hormone-releasing peptide, GHRL,ghrelin,MTLRP; appetite-regulating hormone; ghrelin, growth hormone secretagogue receptor ligand; ghrelin/obestatin preprohormone; growth hormone secretagogue; growth hormone-releasing peptide; motilin-related peptide; prepro-appetite regulatory hormone; ghrelin/obestatin prepropeptide
Search name
Mouse Appetite-regulating hormone ELISA KIT ,Mouse Protein M46 ELISA KIT ,Mouse Motilin-related peptide ELISA KIT ,Mouse Growth hormone secretagogue ELISA KIT ,Mouse Growth hormone-releasing peptide ELISA KIT ,Mouse GHRL ELISA KIT ,Mouse ghrelin ELISA KIT ,Mouse MTLRP ELISA KIT ,Mouse appetite-regulating hormone ELISA KIT ,Mouse ghrelin ELISA KIT ,Mouse growth hormone secretagogue receptor ligand ELISA KIT ,Mouse ghrelin/obestatin preprohormone ELISA KIT ,Mouse growth hormone secretagogue ELISA KIT ,Mouse growth hormone-releasing peptide ELISA KIT ,Mouse motilin-related peptide ELISA KIT ,Mouse prepro-appetite regulatory hormone ELISA KIT ,Mouse ghrelin ELISA KIT,Mouse obestatin prepropeptide ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Appetite-regulating hormone concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Appetite-regulating hormone, During the reaction, Appetite-regulating hormone in the sample or standard competes with a fixed amount of biotin-labeled Appetite-regulating hormone for sites on a pre-coated Monoclonal antibody specific to Appetite-regulating hormone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Appetite-regulating hormone in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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