Mouse T- lymphocyte activation antigen CD80, Cd80 ELISA KIT Product Name:Mouse T- lymphocyte activation antigen CD80, Cd80 ELISA KIT
Packing:96T
Catalog No.:ELI-06512m
Gene Name:Mouse Cd80
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Cd80
Alternative Name:Mouse T-lymphocyte activation antigen CD80, Cd80
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse T- lymphocyte activation antigen CD80, Cd80 ELISA KIT allows for the in vitro quantitative determination of Mouse Cd80 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse T- lymphocyte activation antigen CD80, Cd80 ELISA KIT has been pre-coated with an Mouse T-lymphocyte activation antigen CD80, Cd80 antibody specific to Mouse Cd80 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cd80 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cd80 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cd80 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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