Mouse Interferon regulatory factor 3,IRF3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Mouse IRF3 ELISA KIT ,Mouse IRF-3 ELISA KIT ,Mouse IIAE7 ELISA KIT ,Mouse interferon regulatory factor 3 ELISA KIT
Intended use
Mouse IRF3 ELISA KIT allows for the in vitro quantitative determination of mouse interferon regulatory factor 3,IRF3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IRF3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IRF3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IRF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IRF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 31.2 -2000 pg/ml
Mouse IRF3 ELISA KIT Manual download
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