Mouse Phosphatidylinositol- 3,4,5- trisphosphate 3- phosphatase and dual- specificity protein phosphatase PTEN, PTEN ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PTEN,phosphatidylinositol; 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; phosphatase and tensin-like protein; phosphatase and tensin homolog,3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Search name
Mouse PTEN ELISA KIT ,Mouse phosphatidylinositol ELISA KIT ,Mouse 10q23del ELISA KIT ,Mouse BZS ELISA KIT ,Mouse CWS1 ELISA KIT ,Mouse DEC ELISA KIT ,Mouse GLM2 ELISA KIT ,Mouse MHAM ELISA KIT ,Mouse MMAC1 ELISA KIT ,Mouse PTEN1 ELISA KIT ,Mouse TEP1 ELISA KIT ,Mouse MMAC1 phosphatase and tensin homolog deleted on chromosome 10 ELISA KIT ,Mouse mutated in multiple advanced cancers 1 ELISA KIT ,Mouse phosphatase and tensin-like protein ELISA KIT ,Mouse phosphatase and tensin homolog ELISA KIT,Mouse 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA KIT, Mouse phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, PTEN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTEN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PTEN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PTEN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTEN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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