Mouse Aspartate aminotransferase, cytoplasmic, Got1 ELISA KIT Product Name:Mouse Aspartate aminotransferase, cytoplasmic, Got1 ELISA KIT
Packing:96T
Catalog No.:ELI-04027m
Gene Name:Mouse Got1
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Got1
Alternative Name:Mouse Aspartate aminotransferase, cytoplasmic, Got1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Aspartate aminotransferase, cytoplasmic, Got1 ELISA KIT allows for the in vitro quantitative determination of Mouse Got1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Aspartate aminotransferase, cytoplasmic, Got1 ELISA KIT has been pre-coated with an Mouse Aspartate aminotransferase, cytoplasmic, Got1 antibody specific to Mouse Got1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Got1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Got1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Got1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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