Mouse Nitric oxide synthase, endothelial, Nos3 ELISA KIT Product Name:Mouse Nitric oxide synthase, endothelial, Nos3 ELISA KIT
Packing:96T
Catalog No.:ELI-02753m
Gene Name:Mouse Nos3
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Nos3
Alternative Name:Mouse Nitric oxide synthase, endothelial, Nos3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nitric oxide synthase, endothelial, Nos3 ELISA KIT allows for the in vitro quantitative determination of Mouse Nos3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Nitric oxide synthase, endothelial, Nos3 ELISA KIT has been pre-coated with an Mouse Nitric oxide synthase, endothelial, Nos3 antibody specific to Mouse Nos3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nos3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nos3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nos3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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