pJB866 Catalog No. PVT11390
Packing 2ug
pJB866 Informaiton
Promoter: Pm promoter
Replicon: RK2 oriV
Terminator: Termnator
Plasmid classification: other host plasmids; other plasmids; other functional plasmids.
Plasmid size: 8296bp
Prokaryotic resistance: tetracycline Tet
Cloned strains of Escherichia coli, DH5 A and other Escherichia coli
Culture conditions: 37 centigrade, aerobic, LB
Expression host: Gram-negative bacteria
Training conditions: please refer to the literature
5'sequencing primers: Pm-F:cggtttgatagggataagtcc
3'sequencing primers: Ter-R:ccgcattaaaatctagcgagg
pJB866 Description
PJB866 is a broad host expression vector of Gram-negative bacteria, and Pm promoter drives the expression of target genes.
pJB866 Sequence
LOCUS Exported 8296 bp ds-DNA circular SYN 15-SEP-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8296)
TITLE Direct Submission
JOURNAL Exported Sep 15, 2017
FEATURES Location/Qualifiers
source 1..8296
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 5..127
/label=MCS
promoter complement(133..266)
/label=Pm promoter
/note="The bacterial Pm promoter is activated by XylS in
the presence of benzoate or m-toluate (Marques et al.,
1999)."
CDS 966..1931
/codon_start=1
/gene="xylS"
/product="XylS regulator encoded by the Pseudomonas putida
TOL plasmid pWWO"
/label=XylS
/note="stimulates transcription from the Pm promoter in the
presence of aromatic compounds such as m-toluate or
benzoate"
/translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGPPAGR
LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
oriT 2471..2579
/note="incP origin of transfer"
CDS complement(3036..3686)
/codon_start=1
/label=TetR
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHSTSVPRADDDWRSFLTGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSESGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
CDS 3717..4991
/codon_start=1
/label=TetA
/translation="MSTNLSVIKNPRVQSDQRRLVRRPDVKPNIPLIVILSTVALDAVG
IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALVQFACAPVLGALSDRFGRRPILLVS
LAGATVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSA
CFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREAL
NPLSFVRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG
ILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGI
GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAG
AALYLLCLPALRRGLWSGAGQRADR"
rep_origin 5566..6096
/label=RK2 oriV
/note="incP origin of replication"
misc_feature 6318..6436
/label=Neo Promoter
CDS 6488..7636
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
misc_feature 7687..8296
/label=Termnator
ORIGIN
1 gatcttcgaa tgcatcgcgc gcaccgtacg tctcgaggaa ttcctgcagg atatctggat
61 ccacgaagct tcccatggtg acgtcaccgg ttctagatac ctaggtgagc tctggtaccg
121 cggccgcaat tcacatgttc atgactccat tattattgtt tctgttgcat aaagcctaag
181 gggtaggcct ttctagagat agccattttt tgcactcctg tatccgcttc ttgcaaggct
241 ggacttatcc ctatcaaacc ggacacccgg tcgttggtca gatcaagcta attcccatgc
301 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
361 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
421 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
481 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
541 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgt
601 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
661 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagcagatc ccctttatcc
721 gccaattcgt cgccgcatgc cccgacagca cgaacttctg gtgttctcgc ttcttaaaaa
781 gaacgtcttc gttctgcttg gcgttatttt tgcttggaaa agtggtcact gattgcaaaa
841 aggatggcgc aacgtggcaa tgggggtaac ccgtatacgc atcacgtcga gatgcatttt
901 catcgacttg gcgcctttct acatcacacc aagcagccca cattaaaata agagaaccgt
961 gaactatgga tttttgctta ttgaacgaga aaagtcagat cttcgtccac gccgagccct
1021 atgcagtctc cgattatgtt aaccagtatg tcggtacgca ctctattcgc ctgcccaagg
1081 gcgggccccc ggcaggcagg ctgcaccaca gaatcttcgg atgcctcgac ctgtgtcgaa
1141 tcagctacgg cggtagcgtg agggtaatct cgcctggatt agagacctgt tatcatctgc
1201 aaataatact caaaggccat tgcctgtggc gtggccatgg ccaggagcac tattttgcgc
1261 cgggcgaact attgctgctc aatccggatg accaagccga cctgacctat tcagaagatt
1321 gcgagaaatt tatcgttaaa ttgccctcag tggtccttga tcgggcatgc agtgacaaca
1381 attggcacaa gccgagggag ggtatccgtt tcgccgcgcg acacaatctc cagcaactcg
1441 atggctttat caatctactc gggttagttt gtgacgaagc ggaacataca aagtcgatgc
1501 ctcgggtcca agagcactat gcggggatca tcgcttccaa gctgctcgaa atgctgggca
1561 gcaatgtcag ccgtgaaatt ttcagcaaag gtaacccgtc tttcgagcga gtcgttcaat
1621 tcattgagga gaatctcaaa cggaatatca gccttgagcg gttagcggag ctggcgatga
1681 tgagtccacg ctcgctctac aatttgttcg agaagcatgc cggcaccacg ccgaagaact
1741 acatccgcaa ccgcaagctc gaaagcatcc gcgcctgctt gaacgatccc agtgccaatg
1801 tgcgtagtat aactgagata gccctagact acggcttctt acatttggga cgcttcgctg
1861 aaaactatag gagcgcgttc ggcgagttgc cttccgacac cctgcgtcaa tgcaaaaagg
温馨提示：不可用于临床ZL。
