pKW08-Lx
Catalog No. PVT11355
Packing 2ug
pKW08-Lx Information
Replicon: ColE1 ori
Terminator: rrnB T2 Terminator
Plasmid classification: mycobacterial plasmid
Plasmid size: 7960bp
Plasmid tagging: LuxA, LuxB
Prokaryotic resistance: hygromycin Hygromycin
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic, LB
Expression host: Mycobacterium
5'sequencing primers: Based on full sequence design
pKW08-Lx Sequence
LOCUS Exported 7960 bp ds-DNA circular SYN 30-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pKW08-Lx
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7960)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..7960
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(248..836)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5042..5058
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
terminator 7920..7947
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
ORIGIN
1 actagttcta gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
61 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
1081 ccggacgtgg ccgaccagcc cgtcatcgtc aacgcctgac cgcggtgcgg acaggccgtg
1141 tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg tccgctgtga
1201 cacaagaatc cctgttactt ctcgaccgta ttgattcgga tgattcctac gcgagcctgc
1261 ggaacgacca ggaattctgg gagccgctgg cccgccgagc cctggaggag ctcgggctgc
1321 cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc
1381 ccgacccggt gatcaagctg ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg
1441 agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc
1501 gcggcgagct gcggcccggc accggagcct ggccgtggcc ctacctggtg atgagccgga
1561 tgaccggcac cacctggcgg tccgcgatgg acggcacgac cgaccggaac gcgctgctcg
1621 ccctggcccg cgaactcggc cgggtgctcg gccggctgca cagggtgccg ctgaccggga
1681 acaccgtgct caccccccat tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg
1741 cgaccgtcga ggaccaccgc gggtggggct acctctcgcc ccggctgctg gaccgcctgg
1801 aggactggct gccggacgtg gacacgctgc tggccggccg cgaaccccgg ttcgtccacg
1861 gcgacctgca cgggaccaac atcttcgtgg acctggccgc gaccgaggtc accgggatcg
1921 tcgacttcac cgacgtctat gcgggagact cccgctacag cctggtgcaa ctgcatctca
1981 acgccttccg gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg cagtggaagc
2041 ggaccgagga cttcgcccgc gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt
2101 tcgaggagac cccgctggat ctctccggct tcaccgatcc ggaggaactg gcgcagttcc
2161 tctgggggcc gccggacacc gcccccggcg cctgacgccc cgggccgccc ggcgccgccc
2221 ccggcccccg gcggccgccc ggagccccgc ccgcgctcgg gagccccggg cccgcgccga
2281 agcccgctgc tgcgagcccg gagcgggccg gccgacggcg gtgcgggccc ggcggcggac
2341 gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga tcatctcctc cagggtggtc
2401 ggccgagctt ccatcccagc tcggcaagga tcaacgagga cagtcgcacg acgaagttct
2461 tctggatcgc gcccgtgctg gaagcactca acctcgaagc gtgtggttgc ggagccatct
2521 agcaaccaca cgaaacatgc gcaacgaacc gcgcaacgaa caacgcctag aactggccct
2581 agatgagctg actcgtatcg ttggtaaacc tagtttgacc agcatgtttt aactacgttc
2641 ggtgagctgt caacggggcc tgtaacggca caacgaaccg tgcaacgaga gtggccacgg
2701 atgccaccac aggcactaca acggagttcg ccacgtacat caccacaacc accgattctg
2761 gcggtgagct ccccgatatt cagcggaaat ggcttggtat cgaccaagat tcgtagaacc
2821 ccgtctcgtc tggctggtat tcaaaacggg cgcaacgaaa cacgcaacga gacaggcatg
2881 gcccaaacca gaaaactagc gtctaccagg acttttacct gtccgacccg ttgcaacgga
2941 accccccacg gaacccccgc gacacccgct ccccaattgc gttagaacag cggtggattg
3001 tcggcttcgt tgtgggcctt ttgagccgct tcctgttctg ccgcacgctc tttcctcgcc
3061 cgatagccga gtcgcttaac ggtgtccaga tgcagcccga aatgtttggc cgtttgcggc
3121 caagagtggc cctcgtcgtc gtgataggcg cggatgcgtt cgcggcgtgc agcctgctcg
3181 gcgagccact cgctgcgttc ctgcgccacg agccggacga cgtggcgttc ggatagtccg
3241 gtgattcgag cgccttcggc ggcggtcacg cgccgctttt tgcggacagt cggctgccgg
3301 ttgtagccgt cgctgtagcc gtcgctgtag ccgtcgctca tagcaatgcc tccatggctg
3361 acgcggactt tgcgcgccgc gcaactgtgc tcgccgccgt gcgcgctgct gcgcccttcc
3421 gcgagatggc cgactggcgc gcactgagtg tggcctcgta gaccacgatc ccgtccgccc
3481 aaatgcgcga cttggttgtg atccaacgcc aaatgctgtt ggcgatggcg cggacctcgc
3541 tgtccggtag cggtccggga cacacgtcgt tgcacgggaa ttcggcgttt cgcgcgtggc
3601 actcggcata gatcgcgcgg ccgagtccgt ccacgttccg ggtcggcagg tagatccgca
3661 tgagggcggg acgataggcc cacaacctga cggaatcgaa cagtgcgcaa ttccgcccta
3721 gcggcgtcgg agccgctttg tacgtggtct gctgacgcca gcgcggcggt ggcatgttcg
3781 cgccgagctc ggcctcgatg tggctgagtg tgtagagatc tgagtggagc cattccgttt
3841 cccaggcgat gtggccgggg tttttggtca tgaggcctga gtaactgcgg tcgccgtcga
3901 cggcgcgccg aaggccttcg gcgcacgccg ccatgtatgc gagcggctta cgccgcgcgt
3961 attcggtgcg tggaacaggg gcgttgagtg cccacactgc gtgtgcgtgg ccgttggcgc
4021 gattgcccac gatcgcgttg ggcagcggat gggacccccg ggcgctgagc gctcggagcg
4081 ctgcgtctgg atggtctacg tccacgacca gcaggtttgc cagcgctgtt gggttcgcct
4141 cgatgtaccg gcggcctagg gccgacgcgc ggctttggcg gtagatcccc tcgagcagat
4201 cgtcgcttgc cagcggccag tacggcagcc agagctgctc aaattcgtcg gcgacgtggc
4261 tcacgcttgg tagtagacca cgattaatca ccggtgtatg gtccgacacg agctccaagt
4321 cagatatttc gctgaggggc caccccacaa ctgcacactc ccccgctctc ccgtcgagcc
4381 ctggtggtgg aacaccagcg acagccgagc acccccaacc acctgtacca accaggagga
4441 acacatgcgt cgtttcgagg acgtttccgg gccgctgaga gccgctgtgg cggccgtaca
4501 cgccgcctta gacccgttag accccctgcc gcctgaatgc gcgggtacga gccacacagc
4561 gcccgaactt acggagctgg tgggctcacc tggctttatg gcgtacgaat cggctgtgtg
4621 cgacctgttg ggcgaggtga ggtacgcgct actcacgctg gcaagggcga cacagccgcc
4681 ccaccgagcc cgcacggccg cgcgcggtgt caacaaccgg gtgagtcgtg cacaccagca
4741 ggtgttcgag gcttggctcg aagtgcagga catcgtggcg aacgccgccc gatgagccgc
温馨提示：不可用于临床ZL。
