pBacpAK9
Catalog No. PVT11322
Packing 2ug
Function /
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
pBacpAK9 Description
Available separately or as part of the BacPAK™ Baculovirus Expression System (Cat. No. 631402). pBacPAK9 is a transfer vector designed for high-level expression of a cloned gene driven by the strong AcMNPV polyhedrin promoter. Flanking AcMNPV sequences allow recombination with viral DNA to transfer the expression cassette to the polyhedrin locus of the viral DNA. The polyhedrin coding sequences have been replaced by a multiple cloning site with 18 unique sites which facilitate the insertion of foreign genes in the correct orientation for expression. The Pac I site at the end of the MCS region provides translational stop codons in all three reading frames for expression of truncated proteins. pBacPAK9 has a pUC origin of replication, an M13 origin for single-stranded DNA production, and an ampicillin resistance gene for selection in E. coli.
pBacpAK9 Multiple cloning site
pBacpAK9 Sequence
LOCUS Exported 5538 bp ds-DNA circular SYN 21-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5538
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_recomb 327..1154
/label=baculovirus recombination region (lef2/ORF603)
/note="contains ORF603 and part of lef2"
CDS 327..451
/codon_start=3
/product="baculovirus late expression factor 2"
/label=lef2
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
promoter 1158..1249
/gene="polh from Autographa californica"
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
misc_recomb 1337..2694
/label=baculovirus recombination region (ORF1629)
/note="contains part of ORF1629"
rep_origin 2841..3296
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3578..3682
/gene="bla"
/label=AmpR promoter
CDS 3683..4543
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4714..5302
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatcccgg
1261 gaattcgagc tcggtaccag atcttctaga ttcgaactcg aggcctgcag ggcggccgct
1321 taattaattg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
1381 tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
1441 tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
1501 tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
1561 ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
1621 agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
1681 tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
1741 ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta
1801 ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
1861 tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
1921 gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta
1981 actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac
2041 atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc
2101 ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca
2161 gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta
2221 ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt
2281 ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc
2341 ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct
2401 ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt
2461 ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa
2521 ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac
2581 aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt
2641 aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc
2701 ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc
2761 gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
2821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
2881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
2941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
3001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
3061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
3121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
3181 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
3241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
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