pMT-V5-HisA
Catalog No. PVT11314
Packing 2ug
Function /
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
pMT-V5-HisA Description
pMT/V5-His is a 3.5 kb expression vector designed for use with the Drosophila Inducible Expression System. Upon transfection, the vector allows transient, inducible expression of your protein of interest in Drosophila cells. When cotransfected with the selection vectors, pCoHygro or pCoBlast, included with the appropriate DES® Inducible Kit, pMT/V5-His allows selection of stable cell lines exhibiting inducible expression of the protein of interest.
pMT-V5-HisA Multiple cloning site
pMT-V5-HisA Sequence
LOCUS Exported 3538 bp ds-DNA circular SYN 26-NOV-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3538)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3538
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 407..833
/label=MT promoter
/note="Drosophila metallothionein promoter"
CDS 930..971
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 981..998
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
polyA_signal 1040..1174
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1720..2308)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2479..3339)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3340..3444)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattaatt cgttgcagga
421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagcttt gcatcccata
601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc
841 tagatcgggg tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
901 ggccgctcga gtctagaggg cccttcgaag gtaagcctat ccctaaccct ctcctcggtc
961 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
1021 cctcgactgt gccttctaag atccagacat gataagatac attgatgagt ttggacaaac
1081 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
1141 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
1201 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
1261 tggtatggct gattatgatc agtcgacctg caggcatgca agcttggcgt aatcatggtc
1321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
1381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
1441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
1501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
1561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
1621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
1681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
1741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
1801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
1861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
1921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
1981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
2041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
2101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
2161 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
2221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
2281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
2341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
2401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
2461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
2521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
2581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
2641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
2701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
2761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
2821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
2881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
2941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
3001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
3061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
3121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
3181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
3241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
3301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
3361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
3421 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
3481 aaccattatt atcatgacat taacctataa aaataggcgt atc <
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