pDG1730 Catalog No. PVT11305
Packing 2ug
pDG1730 Sequence
LOCUS Exported 7602 bp ds-DNA circular SYN 27-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7602)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017
FEATURES Location/Qualifiers
source 1..7602
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 115..693
/codon_start=1
/label=amyE-front
/translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP
SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ
PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTH
GNTQIKNWSDRILEAYRIPAALANGDFRS"
CDS 1039..1713
/codon_start=1
/label=Spe
/translation="MFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKI
GDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLY
QAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILT
MDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGENIEWTNENVNLTINYLNNRLK
KL"
CDS 1849..2868
/codon_start=1
/label=amyE-back
/translation="MSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDR
GSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSIN
TATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVT
HSFNDQLTITLRADANTTKAVYQINNGPDDRRLRMEINSQSEKEIQFGKTYTIMLKGTN
SDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMT
KNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH"
CDS 4231..4965
/codon_start=1
/label=Erm
/translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
DLNNISFEQFLSLFNSYKLFNK"
rep_origin complement(5773..6361)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6532..7392)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
601 catggaaaca cacaaattaa aaactggtct gatcggatcc tagaagctta tcgaattcct
661 gcagccctgg cgaatggcga ttttcgttcg tgaatacatg ttataataac tataactaat
721 aacgtaacgt gactggcaag agatattttt aaaacaatga ataggtttac acttacttta
781 gttttatgga aatgaaagat catatcatat ataatctaga ataaaattaa ctaaaataat
841 tattatctag ataaaaaatt tagaagccaa tgaaatctat aaataaacta aattaagttt
901 atttaattaa caactatgga tataaaatag gtactaatca aaatagtgag gaggatatat
961 ttgaatacat acgaacaaat taataaagtg aaaaaaatac ttcggaaaca tttaaaaaat
1021 aaccttattg gtacttacat gtttggatca ggagttgaga gtggactaaa accaaatagt
1081 gatcttgact ttttagtcgt cgtatctgaa ccattgacag atcaaagtaa agaaatactt
1141 atacaaaaaa ttagacctat ttcaaaaaaa ataggagata aaagcaactt acgatatatt
1201 gaattaacaa ttattattca gcaagaaatg gtaccgtgga atcatcctcc caaacaagaa
1261 tttatttatg gagaatggtt acaagagctt tatgaacaag gatacattcc tcagaaggaa
1321 ttaaattcag atttaaccat aatgctttac caagcaaaac gaaaaaataa aagaatatac
1381 ggaaattatg acttagagga attactacct gatattccat tttctgatgt gagaagagcc
1441 attatggatt cgtcagagga attaatagat aattatcagg atgatgaaac caactctata
1501 ttaactttat gccgtatgat tttaactatg gacacgggta aaatcatacc aaaagatatt
1561 gcgggaaatg cagtggctga atcttctcca ttagaacata gggagagaat tttgttagca
1621 gttcgtagtt atcttggaga gaatattgaa tggactaatg aaaatgtaaa tttaactata
1681 aactatttaa ataacagatt aaaaaaatta taaaaaaatt gaaaaaatgg tggaaacact
1741 tttttcaatt tttttgtttt attatttaat atttgggaaa tattcattct aattggtaat
1801 cagattttag aaaacaataa acccttgcat agggggatct cgacatggat gagcgatgat
1861 gatatccgtt taggctgggc ggtgatagct tctcgttcag gcagtacgcc tcttttcttt
1921 tccagacctg agggaggcgg aaatggtgtg aggttcccgg ggaaaagcca aataggcgat
1981 cgcgggagtg ctttatttga agatcaggct atcactgcgg tcaatagatt tcacaatgtg
2041 atggctggac agcctgagga actctcgaac ccgaatggaa acaaccagat atttatgaat
2101 cagcgcggct cacatggcgt tgtgctggca aatgcaggtt catcctctgt ctctatcaat
2161 acggcaacaa aattgcctga tggcaggtat gacaataaag ctggagcggg ttcatttcaa
2221 gtgaacgatg gtaaactgac aggcacgatc aatgccaggt ctgtagctgt gctttatcct
2281 gatgatattg caaaagcgcc tcatgttttc cttgagaatt acaaaacagg tgtaacacat
2341 tctttcaatg atcaactgac gattaccttg cgtgcagatg cgaatacaac aaaagccgtt
2401 tatcaaatca ataatggacc agacgacagg cgtttaagga tggagatcaa ttcacaatcg
2461 gaaaaggaga tccaatttgg caaaacatac accatcatgt taaaaggaac gaacagtgat
2521 ggtgtaacga ggaccgagaa atacagtttt gttaaaagag atccagcgtc ggccaaaacc
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