pYX212
Catalog No. PVT11244
Packing 2ug
PYX212 Description
Promoter: TPI promoter
Plasmid classification: yeast vector, Saccharomyces cerevisiae expression plasmid
Plasmid size: 8346bp
Prokaryotic resistance: ampicillin Amp
Selection marker: URA3
Clone strain: Escherichia coli DH5 alpha
Culture conditions: LB medium, 37 C
The pYX212 plasmid can be expressed in Saccharomyces cerevisiae, and the TPI promoter drives the expression of the target gene. The HA tag is on the C-terminal. WB test can be used to detect the expression of the gene conveniently.
pYX212 Multiple cloning site
pYX212 Sequence
LOCUS Exported 8346 bp ds-DNA circular SYN 05-JUN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pYX212
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8346)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8346
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..48
/note="MCS"
CDS 49..75
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
rep_origin 394..849
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(980..1783)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/note="URA3"
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVLGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVQGERYRKAGWEAYLRRCGQQN"
promoter complement(1784..1999)
/gene="S. cerevisiae URA3"
/note="URA3 promoter"
rep_origin 2853..4195
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
promoter 4484..4588
/gene="bla"
/note="AmpR promoter"
CDS 4589..5449
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLARRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLDNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5620..6208
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 7436..8345
/note="TPI promoter"
ORIGIN
1 gaattcacca tggatcctag ggcccacaag cttacgcgtc gacccgggta tccgtatgat
61 gtgcctgact acgcatgata tctcgagctc agctagctaa ctgaataagg aacaatgaac
121 gtttttcctt tctcttgttc ctagtattaa tgactgaccg atacatccct tttttttttt
181 gtctttgtct agctccagct tttgttccct ttagtgaggg ttaattcaat tcactggccg
241 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
301 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
361 aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg
421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
541 taaatcgggg gctcccttta gggttccgat ttagtggttt acggcacctc gaccccaaaa
601 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
661 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
721 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
781 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
841 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
901 agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
961 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
1021 ctgcttttct gtaacgttca ccctgtacct tagcatccct tccctttgca aatagtcctc
1081 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
1141 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
1201 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
1261 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
1321 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
1381 gcttcaaacc gctaacaata cctggcccca gcacaccgtg tgcattcgta atgtctgccc
1441 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
1501 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
1561 taactgtgcc ctccatcgaa aaatcagtca atatatccac atgtgttttt agtaaacaaa
1621 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
1681 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
1741 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
1801 tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
1861 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
1921 gttcggagat taccgaatca aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa
1981 aaaaaaatga tgaattgaat tgaaaagctg tggtatggtg cactctcagt acaatctgct
2041 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
2101 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
2161 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
2221 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtgc ggccgctcta
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