pLKO.1-Puro
Catalog No. PVT11106
Packing 2ug
pLKO.1-Puro Information
Promoter: hPGK, U6, T7, T3
Replicon: SV40 Ori, F1 Ori
Terminator: SV40 poly (A) signal,
Plasmid classification: virus series, lentivirus interference vector
Plasmid size: 7032bp
Prokaryotic resistance: Amp
Clone strain: Stbl3
Culture conditions: 37 C, aerobic LB
Expression host: lactation cells
Induction mode: no need to induce, transient expression.
5'sequencing primers: U6:ATGGACTATCATATGCTTACCGTA
Primers for 3'sequencing: primers designed based on sequences
pLKO.1-Puro Description
PLKO.1-Puro is a cloning vector of shRNA, originally derived from HIV-1.It contains all the necessary cis-elements for packaging, reverse transcription, and integration for subsequent production of the lentiviral particles.
pLKO.1-Puro Multiple cloning site
pLKO.1-Puro Sequence
LOCUS Exported 7032 bp ds-DNA circular SYN 07-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7032)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, September 7, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..7032
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 529..1128
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
LTR 1256..1489
/note="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 1561..1682
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 1722..1857
/note="SV40 ori"
/note="SV40 origin of replication"
promoter complement(1878..1896)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1906..1922)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2064..2519
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2545..2649
/gene="bla"
/note="AmpR promoter"
CDS 2650..3510
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3681..4269
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4557..4578
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4593..4623
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4631..4647
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4655..4671
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 4692..4710
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 4738..4964
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 4965..5145
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 5192..5317
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5810..6043
/note="RRE"
&nb <
温馨提示：不可用于临床ZL。
