pCS2-EGFP-EB3 Search name
pCS2-EGFP-EB3,Plasmid pCS2-EGFP-EB3,pCS2-EGFP-EB3 vector
pCS2-EGFP-EB3 Information
Promoter: CMV IE94 promoter
Plasmid size: 5634bp
Plasmid label: N-EGFP
Prokaryotic resistance: ampicillin Amp
Species human(H. sapiens)
pCS2-EGFP-EB3 Description
PCS2-EGFP-EB3 is a human gene overexpression plasmid.
pCS2-EGFP-EB3 Sequence
LOCUS Exported 5634 bp ds-DNA circular SYN 11-OCT-2017
DEFINITION synthetic circular DNA
KEYWORDS pCS2-EGFP-EB3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5634)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5634
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 38..1022
/note="CMV IE94 promoter"
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
promoter 1057..1075
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
CDS 1107..1823
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1830..2675
/codon_start=1
/note="EB3"
/translation="MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYC
QFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDN
FEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSP
TGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHEADAQILELNQQLLDLKLTVDGLEKE
RDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY
"
polyA_signal 2705..2839
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3393..3981)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4152..5012)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5013..5117)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(5143..5598)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ggcgatcggt gcgggcctct tcgctattac gccagtcgac catagccaat tcaatatggc
61 gtatatggac tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc
121 gtatatggac tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc
181 ggacttggca ccatgccaat tcaatatggc ggacttggca ctgtgccaac tggggagggg
241 tctacttggc acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata
301 ttgaattggc atggtgccaa taatggcggc catattggct atatgccagg atcaatatat
361 aggcaatatc caatatggcc ctatgccaat atggctattg gccaggttca atactatgta
421 ttggccctat gccatatagt attccatata tgggttttcc tattgacgta gatagcccct
481 cccaatgggc ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc
541 cccattgacg tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc
601 tattgacgta tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct
661 caatgcccat tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc
721 ccattcatat ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg
781 cagtacatca atatctatta atagtaactt ggcaagtaca ttactattgg aaggacgcca
841 gggtacattg gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc
901 aagtacatcc ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga
961 cgtaaatggg cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga
1021 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
1081 agctacttgt tctttttgca ggatccatgg tgagcaaggg cgaggagctg ttcaccgggg
1141 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
1201 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
1261 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
1321 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
1381 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
1441 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
1501 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
1561 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
1621 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
1681 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
1741 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
1801 tcggcatgga cgagctgtac aaggaattca tggctgtcaa tgtgtactcc acttctgtga
1861 ccagtgagaa tctgagtcgc catgatatgc ttgcatgggt caatgactct ctgcacctca
1921 attataccaa gattgaacag ctctgttcag gggcagccta ctgccagttc atggacatgc
1981 tcttccctgg ctgtgttcac ttgaggaagg tcaagttcca ggccaaacta gaacacgagt
2041 acatccacaa cttcaaggtg ctgcaagcag ctttcaagaa gatgggtgtt gacaaaatca
2101 ttcccgtaga gaagttagtg aaaggaaaat tccaagataa ttttgagttt atacagtggt
2161 ttaagaaatt ctttgacgca aactatgatg gaaaggatta caaccctctg ctggcgcggc
2221 agggccagga cgtagcacca cctcctaacc caggtgatca gatcttcaac aaatccaaga
温馨提示：不可用于临床ZL。
