pAdTrack- CMV/ pAdTrack- CMV质粒/ pAdTrack- CMV Plasmid

pAdTrack-CMV

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pAdTrack-CMV,Plasmid pAdTrack-CMV,pAdTrack-CMV vector

 

pAdTrack-CMV Information

Promoter: CMV promoter

Replicon: pUC ori

Terminator: SV40 poly (A) signal

Plasmid size: 9237bp

Prokaryotic resistance: Kan

Selection marker: Neo

Clone strain: Stbl3

Culture conditions: 37 C, aerobic, LB

Induction mode: no induction, instantaneous expression

 

pAdTrack-CMV Description

PAdTrack-CMV transgenic adenovirus vector to cell expression vector with CMV promoter and EGFP gene expression of green fluorescent protein tracking results, and use common pAdeasy1 vector maximum can be inserted into the 5.9kb, the use of common pAdeasy2 carrier can insert the 8.6kb. The specific use of the method can refer to the pAdeasy carrier system. Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We here report a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification.This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.

 

pAdTrack-CMV Multiple cloning site

 

 

pAdTrack-CMV Sequence