pTol2-Promoter-T2A-EGFP Search name
pTol2-Promoter-T2A-EGFP,Plasmid pTol2-Promoter-T2A-EGFP,pTol2-Promoter-T2A-EGFP vector
pTol2-Promoter-T2A-EGFP Information
Promoter: ZB-actin promoter
Replicator: pUC ori, F1 ori, SV40 ori
Plasmid classification: mammalian cells, transposon vectors
Plasmid size: 7312bp
Prokaryotic resistance: Kan
Selection marker: Neo
Clonal strain: DH5 alpha
Culture conditions: 37 C, aerobic LB
Expression host: lactation cells
Induction mode: no need to induce, transient expression.
5'sequencing primers: pEGFP-N-3:CGTCGCCGTCCAGCTCGACCAG
Primers for 3'sequencing: primers designed based on sequences
pTol2-Promoter-T2A-EGFP Cloning site
pTol2-Promoter-T2A-EGFP Sequence
LOCUS Exported 7312 bp ds-DNA circular SYN 07-DEC-2015
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pTol2-Promoter-T2A-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7312)
AUTHORS hp
TITLE Direct Submission
JOURNAL Exported Monday, September 5, 2016 from SnapGene Viewer 3.1.4
COMMENT http://www.informaxinc.com/
ORIGDB|GenBank
FEATURES Location/Qualifiers
source 1..7312
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 12..233
/note="Tol2 5'end"
promoter 262..2926
/note="ZB-actin promoter"
enhancer 2851..2870
/note="PF2Asunbio primer"
exon 2964..3044
/note="2A-peptide"
CDS 3051..3770
/codon_start=1
/note="EGFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 4017..4217
/note="Tol2 3'end"
rep_origin 4228..4683
/note="f1 ori"
rep_origin 5024..5159
/note="SV40 ori"
CDS 5208..6002
/codon_start=1
/note="Kan/Neo"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin 6587..7230
/note="pUC ori"
ORIGIN
1 tagttattaa tagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta
61 ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta
121 cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt
181 caaaaagtac ttattttttg gagatcactt cattctattt tcccttgcta ttagctagcg
241 ctaccggact cagatctcga gcgtttactc tgagaaactt ccatatttta gaggtaaaat
301 gctcagaatt gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag
361 tcacatcacc tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg
421 tttataactc ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca
481 tgtagtttat catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa
541 tatctgaaat gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt
601 tttttttcta cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa
661 aaagaaatgt tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt
721 taagatcaag acttgacata taacatttct aatgaacatt aaacctgagg taggcccact
781 tgcaaaaatg taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa
841 aatagtgaag caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact
901 ttcttaaagt gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa
961 gtagtgtcat cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat
1021 tagatccccc attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag
1081 agctgctgtt gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg
1141 acgcattctg ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc
1201 gttgtcgcag aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt
1261 tataggaaat cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc
1321 aagttgcaag aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc
1381 aatcagagca cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc
1441 atataaaaga gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt
1501 gcggaatatc atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt
1561 gagttggttt tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa
1621 aatgtgtaaa tggataaata tgatttgatg aagtctttag acagttcata acggtctctt
1681 tgtagttatc tactaggttt gatttataag tggtggttct tttgataatc tatgttacat
1741 ttaataaata ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga
1801 atgtcggtaa tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg
1861 tgaaatgggt gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc
1921 ataaatgtga tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg
1981 gatagccggc atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt
2041 agctagccgg ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg
2101 ctcattcgtg ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt
2161 tttaacttgg ttaattttct ttttgttatg tgttctattt aaatgtagcc tgcagccccc
2221 tctgtcttta atgaagctga agattaacgt taacgttagt gaagtgttgt atccgctatg
2281 aaagatttga attttagagg tgtggtccac tttaataacg gctgcggagt tctctggctg
2341 cacttcagcc gctgtgactg tcgaagcaga gcccatcagt ttttcaggat ctcggtgatt
2401 tttgaggcta gactatgaac tgaaccgact gagagtgacc gtccagaaat gctttagtgt
2461 gaagtctccg ctcacgagga gtccgtggac tgactgcaga tctgtgacgc agtaacaccc
2521 ggcggcagac acccgttgaa atgcggttgt gtaattgatc gcaggcgagg gtggaagagg
2581 atgtgaaact tcatttgtgt aaaatttagg gagtggtcct ggtgtgatga atgtcgaaat
2641 ctgttccttt ttactgagcc ctacgactct ggctgagtgc cacaccgccg gcagccgcaa
2701 agcggctcaa accattgcct tttatggtaa taatgagaga atgcagaggg acttcctttg
2761 tctggcatat ctgaggcgcg cgctgtcact agcgcccacc agcggtcaga ctgtagaatg
2821 cagagcaaaa caggaagttg actccagatg gtcacatgct tgctgaaacg cctctccctg
2881 gcagcagtgc aattctaaca gtttcccttt cttttacagt tcagccgaat tctgcagtcg
2941 acggtaccgc gggcccggga tccggaggtg gcagcggtgg cggtcagctg ttgaattttg
3001 accttcttaa acttgcggga gacgtcgagt ccaaccctgg gcccacaacc atggtgagca
3061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
3121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
3181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
3241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
3301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
3361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
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