Search name
pCMV-Tag 2B,Plasmid pCMV-Tag 2B,pCMV-Tag 2B vector
pCMV-Tag 2B Information
Promoter: CMV
Replicator: pUC ori, F1 ori
Terminator: SV40 poly (A) signal, HSV-TK poly (A)
Plasmid classification: mammalian cell carrier; protein overexpression plasmid.
Plasmid size: 4324bp
Plasmid label: N-Flag
Prokaryotic resistance: kanamycin Kan
Screening markers: neomycin Neo
Cloned strain: XL1-Blue
Culture conditions: 37 centigrade, aerobic, LB
Expression host: mammalian cells
Culture conditions: 37 centigrade, aerobic, LB
5'sequencing primers: T3: AATTAACCCTCACTAAAGGG
3'sequencing primers: T7: GTAATACGACTCACTATAGGGC
pCMV-Tag 2B Description
pCMV-Tag 2B Sequence
LOCUS Exported 4324 bp ds-DNA circular SYN 05-11-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled 5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4324)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-11-5
FEATURES Location/Qualifiers
source 1..4324
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 67..370
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 620..638
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 682..705
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
primer_bind complement(753..769)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(826..844)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1118..1239
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1246..1701)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1728..1832
/gene="bla"
/note="AmpR promoter"
promoter 1834..2191
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2042..2177
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2226..3020
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3252..3299
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3628..4216
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc
721 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
781 ggtaccttaa ttaattaagg taccaggtaa gtgtacccaa ttcgccctat agtgagtcgt
841 attacaattc actcgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggagat
901 ccaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
961 tcacagtccc aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat
1021 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
1081 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
1141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
1201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
1261 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
1321 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
1381 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
1441 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
1501 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
1561 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
1621 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
1681 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
1741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
1801 aaatgcttca ataatattga aaaaggaaga atcctgaggc ggaaagaacc agctgtggaa
1861 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
1921 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
