Biotinylated Human CD40 Ligand / TNFSF5 Protein, His Tag, Avi Tag (Avitag™)

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>90% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA. Immobilized a series of concentration of Biotinylated Human CD40 Ligand, His Tag, Avi Tag (Cat. No. CDL-H82Q8) on streptavidin precoated (0.5 μg/well) plate, can bind Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) 2 μg/mL (100 μL/well) with a linear range of 1-40 ng/mL (QC tested).

产品背景:
CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members.CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.

产品描述:
This protein carries a polyhistidine tag at the N-terminus and an Avi tag at the C-terminus. The protein has a calculated MW of 25.6 kDa. The protein migrates as 28 kDa and 30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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