1、机器日程维护和质控很重要，一定要记得使用质控微球
| 产品编号 | 产品名称 | 规格 | 用途 |
| 340486 | BD Calibrite™ 3 Beads | 25T | FACSCalibur 3色质控微球 |
| 340487 | BD Calibrite™ APC Beads | 25T | FACSCalibur APC质控微球 |
| 335775 | BD FACS™ 7-Color Setup Beads | 25T | FACSCanto 7色设置微球 |
| 345249 | BD FACS™ Accudrop Beads | 25T | 分选仪Accdrop微球 |
| 650621 | BD FACSuite™ CS&T Research Beads | 50T | FACSVerse质控微球 |
| 650622 | BD FACSuite™ CS&T Research Beads | 150T | FACSVerse质控微球 |
| 655050 | BD FACSDiva™ CS&T Research Beads | 50T | Diva质控（Diva7及以上） |
| 655051 | BD FACSDiva™ CS&T Research Beads | 150T | Diva质控（Diva7及以上） |
| 641319 | BD™ Cytometer Setup & Tracking Beads Kit | 50T | Diva质控（Diva6.X） |
| 642412 | BD™ Cytometer Setup & Tracking Beads Kit | 150T | Diva质控（Diva6.X） |
| 653144 | BD Accuri Spherotech 8-Peak Beads（FL1-FL3） | 4ml | Accuri质控微球 |
| 653145 | BD Accuri Spherotech 6-Peak Beads（FL4） | 8ml | Accuri质控微球 |
| 556291 | Sphero™ Rainbow Fluorescent Particles, 3.0 - 3.4 µm | 5ml | Rainbow质控微球 |
| 342003 | BD FACSFlow™ Sheath Fluid | 20L | 鞘液 |
| 340345 | BD FACS™ BD FACS Clean Solution | 5L | 清洗液 |
| 334224 | BD FACS™ BD FACS Shutdown Solution | 5L | FACSCanto关机液 |
| 340334 | BD Trucount™ Absolute Counting Tubes | 50T | 计数管 |
| 653160 | BD Accuri™ C6 Flow Cytometer Starter Kit | Kit | Accuri启动套装 |
| 653158 | BD Accuri™ C6 Flow Cytometer Fluid Kit | Kit | Accuri清洗液套装 |
| 653149 | BD Accuri™ C6 Flow Cytometer 1-year Maintenance Kit | Kit | Accuri保养套装（一年） |
| 653150 | BD Accuri™ C6 Flow Cytometer 2-month Maintenance Kit | Kit | Accuri保养套装（2个月） |
| 更多.... | |||
2、样本制备推荐产品：红细胞裂解液和滤网
| 货号 | 产品名称 | 规格 |
| 555899 | Lyse™ Lysing Buffer（10X） | 100ml |
| 352340 | 细胞滤网40μm/蓝色 | 50个/箱 |
| 352350 | 细胞滤网70μm/白色 | 50个/箱 |
| 352360 | 细胞滤网100μm/黄色 | 50个/箱 |
| 更多.... | ||
3、FC受体阻断剂：避免FC非特异性反应
Fc受体是指细胞表面能够与免疫球蛋白(IgG、IgA、IgM、IgE和IgD)集合的分子。其广泛存在于NK细胞、肥大细胞、巨噬细胞、中性粒细胞的表面。FcR能够与抗体的Fc段结合，在检测时产生假阳性。使用FcR阻断剂，可以消除假阳性，降低检测背景，获得更清晰的结果。
| 产品名称 | 产品形式 | 规格 | 产品编号 |
| Human BD FC Block | Purified | 50ug | 564219 |
| 0.25mg | 564420 | ||
| Mouse BD FC Block | Purified | 0.1mg | 553141 |
| 0.5mg | 553142 | ||
| Rat BD FC Block | Purified | 0.1mg | 550270 |
| 0.5mg | 550271 |
4、凋亡试剂盒/周期/增殖
| 产品编号 | 产品名称 | 规格 | 用途 |
| 556547 | FITC Annexin V Apoptosis Detection Kit I | 100T | 凋亡试剂盒（Annexin-FITC,PI） |
| 559763 | PE Annexin V Apoptosis Detection Kit I | 100T | 凋亡试剂盒（Annexin-PE,7-AAD） |
| 550825 | PI/RNase Staining Buffer | 100ml | 细胞周期检测 |
| 559619 | FITC Brd U Flow Kit | 50tests | Brdu增殖试剂盒 |
| 更多... | |||
5、新机器要用起来，准备开始流式实验！选抗体,BD,BD,还是BD.. ！ 可以按照如下搭配好的panel直接选择
常见human Panel搭配
| 亚 群 | 货 号 | 描 述 | 规 格 |
| T 细胞 | 564420 | HU CD4 BB515 | 25T |
| 561949 | HU CD8 PE | 25T | |
| 560835 | HU CD3 PERCP-CY5.5 | 50T | |
| T 细胞 | 560835 | HU CD3 PERCP-CY5.5 | 50T |
| 564419 | Hu CD4 BB515 | 100T | |
| 555367 | HU CD8 PE | 100T | |
| B 细胞 | 561806 | HU CD3 FITC | 25T |
| 561742 | HU CD19 APC | 25T | |
| B 细胞 | 555332 | HU CD3 FITC | 100T |
| 555415 | HU CD19 APC | 100T | |
| NK 细胞 | 561806 | HU CD3 FITC | 25T |
| 561724 | HU CD16 ALEXA 647 | 25T | |
| 561903 | HU CD56 PE | 25T | |
| NK 细胞 | 555332 | HU CD3 FITC | 100T |
| 557710 | HU CD16 ALEXA 647 | 100T | |
| 555516 | HU CD56 PE | 100T | |
| DC | 552764 | HU HLA-DR PERCP-CY5.5 | 50T |
| 560895 | HU CD11C APC | 25T | |
| 561050 | HU CD123 PE | 25T | |
| 565009 | HU CD80 BB515 | 25T | |
| 560957 | HU CD86 PE | 25T | |
| 561960 | HU CD83 APC | 25T | |
| DC | 552764 | HU HLA-DR PERCP-CY5.5 | 50T |
| 559877 | HU CD11C APC | 100T | |
| 555644 | HU CD123 PE | 200T | |
| 565008 | HU CD80 BB515 | 100T | |
| 555658 | HU CD86 PE | 100T | |
| 551073 | HU CD83 APC | 100T | |
| 单核细胞 | 561712 | HU CD14 FITC | 25T |
| 单核细胞 | 555397 | HU CD14 FITC | 100T |
| Treg | 564420 | HU CD4 BB515 | 25T |
| 560987 | HU CD25 APC | 25T | |
| 560082 | HU FOXP3 PE | 25T | |
| 562725 | TRANSCRIPTION FACTOR BUFFER SET 25T | 25T | |
| Treg | 564419 | HU CD4 BB515 | 100T |
| 555434 | HU CD25 APC | 100T | |
| 560046 | HU FOXP3 PE | 100T | |
| 562574 | TRANSCRIPTION FACTOR BUFFER SET 100T | 100T | |
| Treg | 564420 | HU CD4 BB515 | 25T |
| 560987 | HU CD25 APC | 25T | |
| 561028 | HU CD127 PE | 25T | |
| Treg | 564419 | HU CD4 BB515 | 100T |
| 555434 | HU CD25 APC | 100T | |
| 557938 | HU CD127 PE | 100T | |
| TH1/TH2/TH17 | 564420 | HU CD4 BB515 | 25T |
| 560704 | HU IFN-GAMMA NHP PERCP-CY5.5 | 50T | |
| 560671 | HU IL-4 APC | 50T | |
| 560438 | HU IL-17A PE | 25T | |
| 554714 | CYTOFIX/CYTOPERM BUF KIT | 250T | |
| 550583 | LEUKO ACTVTN CKTL WITH GLGPLG 100UL X2 | 200ìL | |
| TH1/TH2/TH17 | 564419 | HU CD4 BB515 | 100T |
| 560704 | HU IFN-GAMMA NHP PERCP-CY5.5 | 50T | |
| 560671 | HU IL-4 APC | 50T | |
| 560436 | HU IL-17A PE | 100T | |
| 554714 | CYTOFIX/CYTOPERM BUF KIT | 250T | |
| 550583 | LEUKO ACTVTN CKTL WITH GLGPLG 100UL X2 | 200ìL | |
| Tfh | 564419 | HU CD4 BB515 | 100T |
| 562781 | HU CXCR5 PERCP-CY5.5 | 50T | |
| 558694 | HU CD279 APC | 100T | |
| 557802 | HU CD278 PE | 100T | |
| 肿瘤干细胞 | 560991 | HU CD24 PE | 25T |
| 560977 | HU CD44 FITC | 25T | |
| 564105 | HU CD45 PERCP-CY5.5 | 100T | |
| 肿瘤干细胞 | 555428 | HU CD24 PE | 100T |
| 555478 | HU CD44 FITC | 100T | |
| 564105 | HU CD45 PERCP-CY5.5 | 100T | |
| 白细胞 | 564106 | HU CD45 PERCP-CY5.5 | 25T |
| 造血干细胞 | 555822 | HU CD34 PE | 100T |
| 红细胞 | 561901 | HU CD55 PE | 25T |
| 560954 | HU CD59 FITC | 25T | |
| 561051 | HU CD23 PE | 25T | |
| 红细胞 | 555694 | HU CD55 PE | 100T |
| 555763 | HU CD59 FITC | 100T | |
| 555570 | HU CD23 PE | 100T | |
| 血小板 | 561913 | HU CD61 FITC | 25T |
| 561921 | HU CD62P PE | 25T | |
| 血小板 | 555753 | HU CD61 FITC | 100T |
| 555524 | HU CD62P PE | 100T | |
| 粒细胞 | 561698 | HU CD13 APC | 25T |
| 561818 | HU CD33 FITC | 25T | |
| 粒细胞 | 557454 | HU CD13 APC | 100T |
| 555626 | HU CD33 FITC | 100T |
更多Panel搭配，欢迎直接咨询优宁维
6、常用mouse Panel搭配
| 亚 群 | 货 号 | 描 述 | 规 格 |
| T细胞 | 561835 | MS CD4 FITC | 25T |
| 561095 | MS CD8A PE | 25T | |
| 561108 | MS CD3E PERCP-CY5.5 | 25T | |
| T细胞 | 553046 | MS CD4 FITC | 100T |
| 553032 | MS CD8A PE | 100T | |
| 551163 | MS CD3E PERCP-CY5.5 | 100T | |
| B细胞 | 561738 | MS CD19 APC | 25T |
| 561878 | MS CD45R PE | 25T | |
| B细胞 | 550992 | MS CD19 APC | 100T |
| 553089 | MS CD45R PE | 100T | |
| NK 细胞 | 557391 | MS NK-1.1 PE | 100T |
| 558759 | MS CD49B PE | 100T | |
| DC树突细胞 | 562363 | MS I-A I-E PERCP-CY5.5 | 50T |
| 550261 | MS CD11C APC | 100T | |
| 561954 | MS CD 80 FITC | 100T | |
| 561963 | MS CD86 PE | 25T | |
| 558206 | MS CD83 APC | 100T | |
| 单核细胞 | 557396 | MS CD11B FITC | 100T |
| 粒细胞 | 553126 | MS LY-6G LY-6C FITC | 100T |
| 565410 | MS F4/80 PE | 100T | |
| Treg细胞 | 561835 | MS CD4 FITC | 25T |
| 561048 | MS CD25 APC | 25T | |
| 563101 | MS FOXP3 PE | 100T | |
| 562725 | TRANSCRIPTION FACTOR BUFFER SET 25T | 25T | |
| Treg细胞 | 553046 | MS CD4 FITC | 100T |
| 557192 | MS CD25 APC | 100T | |
| 563101 | MS FOXP3 PE | 100T | |
| 562574 | TRANSCRIPTION FACTOR BUFFER SET | 100T | |
| TH1/TH2/TH17 | 561835 | MS CD4 FITC | 25T |
| 560660 | MS IFN-GAMMA PERCP-CY5.5 5 | 50T | |
| 562045 | MS IL-4 APC | 25T | |
| 561020 | MS IL-7A PE | 25T | |
| 554714 | CYTOFIX/CYTOPERM BUF KIT | 250T | |
| 550583 | LEUKO ACTVTN CKTL WITH GLGPLG 100UL X2 | 200ul | |
| TH1/TH2/TH17 | 553046 | MS CD4 FITC | 100T |
| 560660 | MS IFN-GAMMA PERCP-CY5.5 | 50T | |
| 554436 | MS IL-4 APC | 100T | |
| 559502 | MS IL-17A PE | 100T | |
| 554714 | CYTOFIX/CYTOPERM BUF KIT | 250T | |
| 550583 | LEUKO ACTVTN CKTL WITH GLGPLG 100UL X2 | 200ul | |
其他常见搭配/辅助试剂
| 种属 | 亚群 | 货 号 | 描 述 | 规 格 |
| rat | Rat T Lymphocyte Cocktail | 558493 | RAT T LYM CKTL | 50T |
| Isotype Control Cocktail B | 558508 | ISOT CTL CKTL B | 20T | |
| Rat Activated T Lymphocyte Cocktail | 558494 | RAT T LYM ACT CKTL | 50T | |
| Rat T/B/NK Cell Cocktail | 558495 | RAT T/B/NK CELL CKTL | 50T | |
| dog | Dog T Lymphocyte Cocktail | 558699 | DOG T LYMPHOCYTE COCKTAIL FITC,PE, APC | 50T |
| Dog Activated T Lymphocyte Cocktail | 558704 | DOG ACT CKTL | 50T | |
| pig | T 细胞 | 559582 | PIG CD3 EPSL FITC | 100T |
| 561474 | PIG CD4A PERCP-CY5.5 | 50T | ||
| 559584 | PIG CD8A PE | 100T | ||
| Isotype control 同型对照 | 556649 | FITC Mouse IgG1, ê Isotype Control | 50T | |
| 552834 | PerCP-Cy5.5 Mouse IgG1 ê Isotype Control | 50T | ||
| 550854 | APC Mouse IgG1 ê Isotype Control | 50T | ||
| 556652 | FITC Mouse IgG2a, ê Isotype Control | 50T | ||
| 556655 | FITC Mouse IgG2b, ê Isotype Control | 50T | ||
| 552916 | FITC Rat IgG1 ê Isotype Control | 50T | ||
| 551979 | PE Rat IgG1, ê Isotype Control | 50T | ||
| 560537 | PerCP-Cy5.5 Rat IgG1, ê Isotype Control | 0.1MG | ||
| 554686 | APC Rat IgG1, ê Isotype Control | 0.1MG | ||
| NA/LE抗体 | 555336 | Purified NA/LE Mouse Anti-Human CD3 HIT3a | 0.5MG | |
| 555725 | Purified NA/LE Mouse Anti-Human CD28 CD28.2 | 0.5MG | ||
| 553057 | Purified NA/LE Hamster Anti-Mouse CD3e 145-2C11 | 0.5MG | ||
| 553294 | Purified NA/LE Hamster Anti-Mouse CD28 37.51 | 0.5MG |
7、细胞因子检测看过来，BD CBA多因子检测方法省钱、省时间、省样本
——50ul样本Z多检测30多种细胞因子，有了流式细胞仪，方便！
CBA经典产品
| 产品名称 | 产品编号 | 检测指标 | 包装 规格 |
| Human Inflammatory Cytokine Kit | 551811 | IL-8, IL-1β, IL-6, IL-10, TNF, IL-12p70 | 80T |
| Human Th1/Th2 Cytokine Kit II | 551809 | IL-2, IL-4, IL-6, IL-10, TNF, IFN-g | 80T |
| Human Th1/Th2/Th17 Kit | 560484 | IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ, IL-17A | 80T |
| Mouse Inflammation Kit | 552364 | L-6, IL-10, MCP-1, IFN-γ, TNF, IL-12p70 | 80T |
| Mouse Th1/Th2/Th17 Cytokine Kit | 560485 | IL-2, IL-4, IL-6, IFN-γ, TNF, IL-17A, IL-10 | 80T |
| Mouse Th1/Th2 Cytokine Kit | 551287 | IL-2, IL-4, IL-5, IFN-γ,TNF | 80T |
| Non-human Primate Th 1 , /Th 2 Cytokine Kit | 557800 | IL-2, IL-4, IL-5, IL-6,TNF, IFN-γ | 80T |
| 更多..... | |||
8、磁珠分选和分选型仪器仪器使用更配哦，超高性价比！
磁珠分选经典产品：
| 目的细胞 | 产品编号 | 产品名称 | 规格 |
| Human T细胞 | 557874 | Hu T Lymphocyte Enrichment Set - DM | set |
| Human CD4+ T细胞 | 557939 | Hu CD4 T Lymphocyte Enrichment Set - DM | set |
| Human CD8+T细胞 | 557941 | Hu CD8 T Lymphocyte Enrichment Set - DM | set |
| Human B细胞 | 558007 | Hu B Lymphocyte Enrichment Set -DM | set |
| Human NK细胞 | 557987 | Hu NK Cell Enrichment Set - DM | set |
| Mouse T细胞 | 557793 | Ms T Lymphocyte Enrichment Set -DM | set |
| Mouse CD4+T细胞 | 558131 | Ms CD4 T Lymphocyte Enrichment Set - DM | set |
| Mouse CD8+T细胞 | 558471 | Ms CD8 T Lymphocyte Enrichment Set - DM | set |
| Mouse B细胞 | 557792 | Ms B Lymphocyte Enrichment Set -DM | set |
| Mouse NK细胞 | 557954 | Ms NK Cell Enrichment Set - DM | set |
| Mouse DC树突细胞 | 557955 | Ms Dendritic Cell Enrichment Set - DM | set |
| PE间标磁珠 | 557899 | Anti-PE Magnetic Particles - DM E31-1459 | set |
| APC间标磁珠 | 557932 | APC Magnetic Particles - DM | set |
| 链酶亲和素间标磁珠 | 557812 | Streptavidin Particles Plus - DM | set |
| 磁珠分选用buffer | 55, 2362 | Buffer (10x) for use with BD IMag ™cell separation products | set |
9、补偿微球
由于荧光光谱的重叠现象，在进行流式多色分析时，必须设置单染管进行荧光补偿。OneComp/UltraComp eBeads微球大小和淋巴细胞相当，可连接各种荧光标记的抗体来调节补偿。每滴微球中都含有两群：一群是可以结合抗体的阳性群，一群是不会与抗体反应的阴性群。
优点：
•使用方便，一滴即可
•与所有小鼠、大鼠和仓鼠来源的抗体反应，要求抗体来源是Kappa
•适用于各种激发光—适用于488nm蓝光，532nm绿光，561nm黄光，633-635nm红光，UltrComp eBeads尤其适用紫外紫色（405nm）激光器。
1）CompBead Compensation Particles 通用补偿微球
| 产品编号 | 产品名称 | 规格 | 反应 | 适合激光器 |
| 552844 | Anti-rat Ig, k | 6ml | 适用于rat来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), 和红色 (633-635 nm)激光器 |
| 552845 | Anti-rat/hamster Ig, k | 6ml | 适用于rat/hamster来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), 和红色 (633-635 nm)激光器 |
| 552843 | Anti-mouse Ig, k | 6ml | 适用于mouse来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), 和红色 (633-635 nm)激光器 |
2）CompBead Plus Particles 补偿大微球，适用于larger cell types, for example, human embryonic stem cells (hESCs)
| 产品编号 | 产品名称 | 规格 | 反应 | 适合激光器 |
| 560499 | Anti-rat Ig, k | 6ml | 适用于rat来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), 和红色 (633-635 nm)激光器 |
| 560497 | Anti-mouse Ig, k | 6ml | 适用于mouse来源的一抗 | 蓝(488 nm), 绿(532 nm), 黄绿 (561 nm), 和红色 (633-635 nm)激光器 |
10、细胞死活鉴定染料 Fxiable Viability Stain(FVS)Reagents
为什么要鉴定细胞死活？
• 死细胞非特异性染色使细胞背景ZG，影响检测灵敏度
• 鉴定细胞生命活性
• 死细胞假阳性染色结果影响真实实验结果比例
与传统染料PI、7-AAD相比的优势？适用于胞内染色的死活细胞鉴定
①、PI、7-AAD的染色通常是放在其他抗体孵育完成后进行，不能洗涤即要上机检测；
②、对于胞内抗体的染色，由于经过了固定步骤，PI或7-AAD染色后所有的细胞都有阳性，所以它们只适合胞膜染色的死活细胞鉴定。
③、PI、7-AAD与常用染色通道PE、PercP是统一检测通道，二者只能选择其一使用。PI和7-AAD发射谱很宽，488nm蓝色激光发射基本上580BP和690BP都被占用，这样大大减小多色试验中的配色增加了调补偿的难度。
④、Fixable Viability Dye的优点是：
—固定、破膜以及洗涤步骤不影响Fixable Viability Dye的染色
—FVS系列光谱多样，适合各种染色组合的搭配。FVS系列分别对应不同的激光器，发射波长不同，波谱集中。
| 产品编号 | 产品名称 | 规格 | 所在荧光通道 |
| 562247 | Fxiable Viability Stain450 | 紫激光 | BV421/V450 |
| 564406 | Fxiable Viability Stain510 | BV | |
| 564407 | Fxiable Viability Stain520 | 蓝激光 | 适用于mouse来源的一抗 |
| 564995 | Fxiable Viability Stain570 | 适用于mouse来源的一抗 | |
| 564996 | Fxiable Viability Stain620 | 适用于mouse来源的一抗 | |
| 564996 | Fxiable Viability Stain620 | 黄/绿激光 | 适用于mouse来源的一抗 |
| 564405 | Fxiable Viability Stain660 | 红激光 | 适用于mouse来源的一抗 |
| 564997 | Fxiable Viability Stain700 | 适用于mouse来源的一抗 |
Fixable Viability Dye使用原理
FVS为氨基反应性染料，与细胞表面或者内部的蛋白不可逆的结合。FVS是染细胞表面或膜内侧的游离胺，活细胞弱阳。正是由于和PI、7-AAD原理不同，所以对于一些需要固定破膜步骤的试验FVS一样适用，如果固定破膜后细胞发生死亡，该染料还是能反应细胞的死活情况，这点是核酸染料所不能替代的。
