我们的Bac-to-Bac 杆状病毒表达系统提供了一种快速、QL、可靠的方法来制备重组杆状病毒，从而在昆虫细胞中表达感兴趣的基因。经基因工程改造的pFastBac™载体与TOPO平末端克隆技术结合在一起可将你的克隆反应简化至仅需5分钟的实验台反应，相比而言传统的限制性酶切克隆方法则需要花费大量时间，通常都需要2至3天。
Bac-to-Bac表达系统的优势：
Advantages of the Bac-to-Bac Expression Systems:
Download the Bac-to-Bac Expression Kit Handbook
Bac-to-Bac method delivers high titersTraditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. In contrast, the Bac-to-Bac Baculovirus Expression System is faster and easier because the technology relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells, which often results in low titers.
Our pFastBac™ TOPO Cloning vector recombines with the parent bacmid in DH10Bac™ E. coli competent cells to form an expression bacmid, which is then used for transfection into insect cells. After the very first transfection of the bacmid, you will produce high titers of pure recombinant baculovirus particles, saving weeks of precious time and enabling large-scale protein expression experiments.
Multiple kit formats for your convenienceWe offer two formats of our Bac-to-Bac Baculovirus Expression Systems. The Expression system format in the most complete kit available; these kits include pFastBac™ TOPO cloning vector, fast competent cells for cloning, specialized competent cells bacmid generation and our CellFectin II Transfection reagent. If you simply need the pFastBac™ vector, we offer a Cloning kit format, which contain the pFastBac™ TOPO vector and competent cells for cloning in the gene of interest.
订购信息| Catalog # | Name | Size | 单价 (CNY) | Qty | |
|---|---|---|---|---|---|
| A11099 | Bac-to-Bac™ N-His TOPO™ Cloning Kit | 20 reactions | 22,599.00 | ||
| A11098 | Bac-to-Bac™ C-His TOPO™ Cloning Kit | 20 reactions | 22,854.00 | ||
| A11100 | Bac-to-Bac™ C-His TOPO™ Expression System | 20 reactions | 48,041.00 | ||
| 10361012 | MAX Efficiency™ DH10Bac Competent Cells | 5 x 100 µL | 8,097.00 | ||
| A11338 | Bac-to-Bac™ HBM TOPO™ Cloning Kit | 20 reactions | 27,872.00 | ||
| A11339 | Bac-to-Bac™ HBM TOPO™ Secreted Expression System | 20 reactions | 51,080.00 | ||
| Catalog # | Name | Size | Qty |
|---|---|---|---|
| 11496015 | Sf9 cells in Sf-900™ II SFM | 1.5 mL | |
| B82501 | Sf9 Cells in Grace's | 1 mL | |
| 11497013 | Sf21 cells in Sf-900™ II SFM | 1.5 mL | |
| B82101 | Sf21 Cells in Grace's | 1 mL | |
| 12682019 | Sf21 cells in Sf-900™ III SFM | 1 vial | |
| B85502 | High Five™ Cells in Express Five™ Medium | 3 x 106cells | |
| 12552014 | Mimic™ Sf9 Insect Cells | 1 mL | |
| 10902096 | Sf-900™ II SFM | 500 mL | |
| 10486025 | Express Five™ SFM | 1000 mL | |
| 11605102 | Grace's Insect Medium, supplemented | 10 x 500 mL | |
| 25030081 | L-Glutamine (200 mM) | 100 mL |
