Hypoxyprobe (Pimonidazole Hydrochloride） 缺氧探针

答：Varghese et al. showed that hypoxiccells bind 2-nitroimidazoles to peptide thiols such as glutathione. The currentview of the metabolism of pimonidazole is summarized in the scheme below. O2competes for the addition of the first electron to pimonidazole which accountsfor the pO2 dependence of activation and binding. On the basis of test tubeexperiments, it is estimated that ca 20 % of activated pimonidazole binds tocellular thiols and ca 80% does not bind but is fragmented by reaction withwater. Pimonidazole is subject to oxidative metabolism leading to easilyexcretable N-oxide, sulfate and glucuronate derivatives (ST = sulfotransferase;GT = glucuronly transferase）. These oxidative pathways do not appear tointerfere with the utility of pimonidazole as a hypoxia marker.  电话：  是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。