二.Rat BioActive Intact PTH ELISA Kit Enzyme-linked ImmunoSorbent Assay （ELISA）for the Quantitative Determination of Rat Bioactive Intact Parathyroid Hormone Levels in Plasma or Cell Culture Media.
三.Rat BioActive Intact PTH ELISA Kit INTENDED USE
This kit is intended for research use only in the determination of rat bioactive intact PTH levels in plasma or cell culture media.
四.Rat BioActive Intact PTH ELISA Kit INTRODUCTION
Rat intact parathyroid hormone （PTH）is an 84 amino acid polypeptide produced by the parathyroid gland with its biological activity residing in the N-terminal region of the peptide. PTH plays an important role in maintaining the concentration of ionized calcium within the limits needed to achieve normal metabolic functions. When serum calcium levels are decreased the parathyroid gland increases secretion of the hormone which results in increased mobilization of calcium from skeletal reserves into the circulation. When levels of serum calcium are increased the secretion of PTH is reduced. The similarities between rat and human physiology relative to calcium metabolism make rat an excellent live-animal model for studying human skeletal disease and in the pre-clinical evaluation of pharmacologic agents that may alter bone remodeling. Quantitation of only full-length biologically active rat intact PTH with this kit can provide a precise and sensitive assessment of changes in bone and mineral metabolism.
五.Rat BioActive Intact PTH ELISA Kit TEST PRINCIPLE
The Rat BioActive Intact PTH ELISA Kit is a two-site enzyme-linked immunosorbent assay （ELISA）for the measurement of PTH in rat plasma or cell culture media. Two affinity purified goat polyclonal antibodies have been selected to detect only the full-length biologically active intact form of rat PTH. The antibody which recognizes epitopes within the C-terminal portion of the peptide is biotinylated for capture. The other antibody which recognizes the initial N-terminal epitope is conjugated with the enzyme horseradish peroxidase （HRP）for detection.
