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HTB-125 Hs 578Bst 人正常乳腺细胞 Hs 578Bst 人正常乳腺细胞

产品信息
简单介绍HTB-125 Hs 578Bst 人正常乳腺细胞, ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和优培养条件！



HTB-125 Hs 578Bst 人正常乳腺细胞 的详细介绍




Organism

Homo sapiens, human

Tissue

mammary gland/breast

Cell Type

Epithelial, Myoepithelial

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

1

Disease

normal

Age

74 years

Gender

female

Ethnicity

Caucasian


HTB-125 Hs 578Bst 人正常乳腺细胞


Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 46; range = 42 to 48

This is a diploid human cell line with 46,XX karyotype. Polyploidy occurred at 6.9%. Both X chromosomes were normal. The chromosome N9 pair was heteromorphic for the centromeric heterochromatin having one with the normal size and the other about twice the size of the normal.


Derivation


Hs 578Bst was derived by A.J. Hackett, et al. from normal breast tissue peripheral to an infiltrating ductal carcinoma which was the source for Hs 578T (see ATCC HTB-126).



Hs 578Bst may have been myoepithelial in origin since the cells possessed microfilaments and clusters of pinocytotic vesicles similar to those seen in myoepithelia in vivo.


Receptor expression

Epidermal growth factor (EGF)


HTB-125 Hs 578Bst 人正常乳腺细胞


Comments


No desmosomes were observed, estrogen receptor protein was not present, and no endogenous viruses were detected.



A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximately 22 more population doublings before the onset of senescence. See Batch Specific information for PDL of current Distribution freeze.


Complete Growth Medium

The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: 30 ng/ml mouse EGF; fetal bovine serum to a final concentration of 10%.

Subculturing


Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.




  1. Remove and discard culture medium.


  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.


  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.


  5. Add appropriate aliquots of the cell suspension to new culture vessels.


  6. Incubate cultures at 37°C.



Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week



HTB-125 Hs 578Bst 人正常乳腺细胞



Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.


Cryopreservation

Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.


HTB-125 Hs 578Bst 人正常乳腺细胞


Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%



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