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Chicken OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F24271

产品信息
  • Chicken OPG / Osteoprotegerin ELISA Kit (Sandwich ELISA) - LS-F24271Catalog
    Size
    Price
    LS-F24271-1
    1 plate
    $725 USD
    Available Discounts
    5% off 5+ units
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    SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F24271 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Chicken OPG / Osteoprotegerin in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of OPG / Osteoprotegerin as low as 75 picograms per milliliter.
    SpecificationsType
     
    Sandwich ELISA (enzyme-linked immunosorbent assay) kit
    Target
     
    OPG / Osteoprotegerin
    Reactivity
     
    Chicken
    Specificity
     
    This kit recognizes natural and recombinant Chicken OPG.
    Manual
     

    Intended Sample Types
     
    Plasma, Serum
    Format
     
    96-Well Microplate
    Detection
     
    Colorimetric - 450nm (TMB)
    Measurement
     
    Quantitative
    Detection Range
     
    125 - 8000 pg/ml
    Sensitivity
     
    75 pg/ml
    Assay Time
     
    3.5 h
    Precision
     
    Intra-Assay: CV<10% Inter-Assay: CV<10%
    Storage
     
    Short term: 4°C; Long term: see manual.
    Quality Assurance
     
    Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
    Kit Components
     
    • User Manual
    • 96-well Microplate
    • Detection Reagents
    • Wash Buffer
    • Substrate
    • Stop Solution
    • Adhesive Plate Sealers

    Protocol
     
    1. Prepare all reagents, samples and standards.
    2. Add 100 µl standard or sample to each well and incubate for 90 minutes at 37°C.
    3. Aspirate and add 100 µl prepared Detection Antibody and incubate of 1 hour at 37°C.
    4. Aspirate and wash 3 times.
    5. Add 100 µl HRP Conjugate and incubate for 30 minutes at 37°C.
    6. Aspirate and wash 5 times.
    7. Add 90 µl Substrate Solution and incubate for 15-20 minutes at 37°C.
    8. Add 50 µl Stop Solution.
    9. Read immediately at 450nm.

    Restrictions
     
    For research use only.
    Guarantee
     
    This elisa kit carries the LSBio Guarantee.
    About OPG / Osteoprotegerin
     
    O00300  NM_002546 NP_002537.3

     

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    Sandwich ELISA Platform Overview
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