Human Fibronectin / Fibronectin Fragment 2 Protein (His Tag)

分子别名（Synonym）:CIG,ED-B,FINC,FN,FNZ,GFND,GFND2,LETS,MSF种属（Species）：Human表达系统（Expressed Host）：HEK293 Cells制剂（Buffer）：Lyophilized from sterile PBS, pH 7.2分子量（Molecule Mass）：The secreted recombinant human Fibronectin 1 fragment2 (FN1.2) consists of 670 amino acids and has a calculated molecular mass of 73.2 kDa. As a result of glycosylation, rhFN1.2 migrates as an approximately 85-100 kDa band in SDS-PAGE under reducing conditions.内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method.生物活性(Activity)：Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well areadded to CD4-coated plates (1.25μg/mL and 100μL/well),approximately 50%-80% will adhere specifically after 30 minutes at 37℃.表达区间（Protein Construction）：A DNA sequence encoding the Fragment 2 (Ser 607-Pro 1265) of humanFibronectin (NP_997639.1) was expressed with a C-terminal polyhistidine tag.存储条件（Storage）：For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution.运输条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.