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Luciferase from Photinus pyralis (firefly), Recombinant

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  • Website:https://www.creative-enzymes.com/product/luciferase-from-photinus-pyralis-firefly-recombinant_1287.html;Description:Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.;Source:E. coli;Species:Photinus pyralis (firefly);Form:Type I, Supplied as a solution in 50 mM Tris HCl pH 8.0, 1 mM dithiothreitol, 1 mM EDTA, and 10% (v/v) glycerol; Type II, Lyophilized powder approximately 20% protein; balance is primarily NaCl, HEPES buffer salts, and carbohydrate..;Enzyme Commission Number:EC 1.13.12.7;Activity:Type I , > 5 ,000 ,000 units/mg protein; Type II, 15-30×106 light units/mg protein.;Unit Definition:One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.;Synonyms:Photinus-luciferin 4-monooxygenase (ATP-hydrolysing); firefly luciferase; luciferase (firefly luciferin); Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing); firefly luciferin luciferase; Photinus pyralis luciferase; EC 1.13.12.7; 61970-00-1;Purity:;Inhibitors:;了解更多产品请留言,或 直接与孚博生物销售人员联系联系人: 邢先生 电话:13260236521 | 固话:010-56146401 | QQ:20493615 | Email:info@f-biology.com
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