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Transglutaminase 1 from Human keratinocyte, Recombinant

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  • Website:https://www.creative-enzymes.com/product/transglutaminase-1-from-human-keratinocyte-recombinant_16118.html;Description:Transglutaminase is based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHMDGPR. Transglutaminase is purified by IMAC to more than 90 % purity.;Source:E. coli;Species:Human;Form:The transglutaminase is lyophilized from 50 mM Tris-HCl pH 8.0, 10 mM Glutathion.;Enzyme Commission Number:EC 2.3.2.13;Activity:> 2,500 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after transglutaminase-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)].;Unit Definition:1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].;Synonyms:transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase; TG1;Purity:> 90 % (visually by SDS-PAGE);Inhibitors:;了解更多产品请留言,或 直接与孚博生物销售人员联系联系人: 邢先生 电话:13260236521 | 固话:010-56146401 | QQ:20493615 | Email:info@f-biology.com
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