Human NR3C1/Glucocorticoid Receptor ELISA Kit (Competitive EIA) - LS-F39288Catalog Size Price LS-F39288-1 1 plate $715 USD Request Bulk Add to Wish List Add to Cart SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F39288 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human NR3C1/Glucocorticoid Receptor in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of NR3C1/Glucocorticoid Receptor as low as 18.75 picograms per milliliter.SpecificationsType
Competitive EIA (enzyme immunoassay) kit Target
NR3C1/Glucocorticoid Receptor Reactivity
Human Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
31.2 - 2000 pg/ml Sensitivity
18.75 pg/ml Precision
Intra-Assay: CV<8% Inter-Assay: CV<10% Storage
Store at 4°C. Stable for 6 months. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with NR3C1. During the reaction, NR3C1 in the sample or standard competes with a fixed amount of NR3C1 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to NR3C1. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NR3C1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About NR3C1/Glucocorticoid Receptor
P04150 NM_000176 NP_000167.1
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