Mouse/Human/Rat Phospho-MAPKAPK2 / MAPKAP Kinase 2 (Thr334) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1046Catalog Size Price LS-F1046-1 1 plate $740 USD Request Bulk Add to Wish List Add to Cart SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F1046 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human MAPKAPK2 / MAPKAP Kinase 2 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.SpecificationsType
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
User Manual
96-well Cell Culture Microplate
Blocking Buffer
Detection Antibodies
HRP-Conjugate
Substrate
Stop Solution
Crystal Violet Solution
SDS Solution
Adhesive Plate Sealers
Protocol
Seed cells into wells and incubate overnight at 37°C, 5% CO2.
Apply desired treatment conditions.
Add 100 µl of Fixing Solution and incubate 20 minutes at room temperature.
Add 100 µl of Quenching Buffer and incubate 20 minutes at room temperature.
Add 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
Add 50 µl of 1x primary antibodies and incubate overnight at 4°C. Add 50 µl of HRP-conjugated secondary antibodies and incubate for 1.5 hours at room temperature.
Add 50 µl of Ready-to-Use Substrate and incubate for 30 minutes at room temperature.
Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About MAPKAPK2 / MAPKAP Kinase 2
P49137 NM_004759 NP_004750.1
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