基本信息
| 启动子: | 35S promoter |
| 原核抗性: | 氨苄青霉素Amp |
| 克隆菌株: | 大肠杆菌Stbl3 |
| 5'测序引物: | 35S: CTATCCTTCGCAAGACCCTTC |
质粒简介
pHANNIBAL是一个植物细胞RNAI干扰质粒质粒图谱
质粒序列
LOCUS Exported 5826 bp ds-DNA circular SYN 03-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5826
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(19..35)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(215..643)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 670..774
/gene="bla"
/label=AmpR promoter
CDS 775..1635
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1806..2394
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 2865..4208
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
intron 4236..5004
/gene="pdk"
/number="3"
/label=PDK intron
/note="pyruvate orthophosphate dikinase intron from
Flaveria trinervia"
terminator 5051..5757
/label=OCS terminator
/note="octopine synthase terminator"
promoter complement(5798..5816)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat
181 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
481 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
1021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
1081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
1141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
1201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
1261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
1321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
1381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
1441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
1501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
1561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
1621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
1681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
1741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
1801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
1861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
1921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
1981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
2041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
2221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
2281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
2341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
2401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
2461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
2521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
2581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
2641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
2701 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
2761 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt tggccaagtc
2821 ggcctctaat acgactcact atagggagct cgtcgagcgg ccgctcgacg aattaattcc
2881 aatcccacaa aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat
2941 tcaacaccct catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga
3001 tgactggggt tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt
3061 tgccactatt acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga
3121 caggttgaac ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc
3181 cctaacaagc ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag
3241 cagtgatcca gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct
3301 ctatctttac gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac
3361 tgataatgag aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag
3421 agaggcctac gcagcaggtc tcatcaagac gatctacccg agtaacaatc tccaggagat
3481 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa
3541 gaacacagag aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca
3601 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc
3661 tactgaatct aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg
3721 ccgtgaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa
3781 tcttcgtcaa catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag
3841 tctcagaaga ccaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc
3901 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg
3961 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg
4021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
4081 caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg
4141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
4201 agaggacacg ctcgaggaat tcggtacccc aattggtaag gaaataatta ttttcttttt
4261 tccttttagt ataaaatagt taagtgatgt taattagtat gattataata atatagttgt
4321 tataattgtg aaaaaataat ttataaatat attgtttaca taaacaacat agtaatgtaa
4381 aaaaatatga caagtgatgt gtaagacgaa gaagataaaa gttgagagta agtatattat
4441 ttttaatgaa tttgatcgaa catgtaagat gatatactag cattaatatt tgttttaatc
4501 ataatagtaa ttctagctgg tttgatgaat taaatatcaa tgataaaata ctatagtaaa
4561 aataagaata aataaattaa aataatattt ttttatgatt aatagtttat tatataatta
4621 aatatctata ccattactaa atattttagt ttaaaagtta ataaatattt tgttagaaat
4681 tccaatctgc ttgtaattta tcaataaaca aaatattaaa taacaagcta aagtaacaaa
4741 taatatcaaa ctaatagaaa cagtaatcta atgtaacaaa acataatcta atgctaatat
4801 aacaaagcgc aagatctatc aattttatat agtattattt ttcaatcaac attcttatta
4861 atttctaaat aatacttgta gttttattaa cttctaaatg gattgactat taattaaatg
4921 aattagtcga acatgaataa acaaggtaac atgatagatc atgtcattgt gttatcattg
4981 atcttacatt tggattgatt acagttggga agctgggttc gaaatcgata agcttggatc
5041 ctctagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt
5101 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg
5161 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt
5221 ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca
5281 atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca
5341 attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa
5401 aagactgatt acataaatct tattcaaatt tcaaaaggcc ccaggggcta gtatctacga
5461 cacaccgagc ggcgaactaa taacgttcac tgaagggaac tccggttccc cgccggcgcg
5521 catgggtgag attccttgaa gttgagtatt ggccgtccgc tctaccgaaa gttacgggca
5581 ccattcaacc cggtccagca cggcggccgg gtaaccgact tgctgccccg agaattatgc
5641 agcatttttt tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg
5701 acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac
5761 ctgcaggcat gcaagctagc ttactagtga tgcatattct atagtgtcac ctaaatctgc
5821 ggccgc
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