pEarleyGate 100植物表达质粒

基本信息

原核抗性:	Kan
筛选标记:	Bar
克隆菌株:	DB3.1
培养条件:	LB/37℃

质粒简介
        pEarleyGate 100植物表达质粒质粒图谱
质粒序列
LOCUS       Exported               11649 bp ds-DNA     circular SYN 06-JUL-2018
DEFINITION  Gateway(R)-compatible plant transformation vector with no encoded 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pEarleyGate 100
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 11649)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, July 6, 2018 
COMMENT     The sequence was corrected by inserting a G at position 7757.
FEATURES             Location/Qualifiers
     source          1..11649
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /note="MAS terminator"
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="Bar"
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /note="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2663..2782
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2812..2842
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2896..3555
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3897..4202
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4243..4367)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4420..5127
                     /note="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    5371..5395
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6695..7324
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7753..8826
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8892..9086
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9430..9570
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9756..10344)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10431..11225)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     2701 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     2761 taaaacacaa catatccagt catattggcg gccgcattag gcaccccagg ctttacactt
     2821 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     2881 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     2941 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     3001 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     3061 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     3121 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     3181 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     3241 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     3301 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     3361 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     3421 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     3481 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     3541 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     3601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     3661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     3721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     3781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     3841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     3901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     3961 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     4021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     4081 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     4141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     4201 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     4261 tgtgttttac agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     4321 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc taggtgagtc
     4381 tagagagtta attaagaccc gggactagtc cctagagtcc tgctttaatg agatatgcga
     4441 gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg
     4501 agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc
     4561 gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg taccctacta
     4621 cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca
     4681 tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta
     4741 aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt
     4801 caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac
     4861 tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt
     4921 ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg
     4981 gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa
     5041 tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt
     5101 ttgcgacaac atgtcgaggc tcagcaggac ctgcaggcat gcaagcttgg cactggccgt
     5161 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5221 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5281 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     5341 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     5401 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
     5461 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     5521 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     5581 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     5641 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     5701 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     5761 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     5821 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     5881 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     5941 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     6001 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     6061 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     6121 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     6181 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     6241 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     6301 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     6361 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     6421 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     6481 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     6541 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     6601 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     6661 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     6721 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     6781 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     6841 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     6901 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     6961 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     7021 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     7081 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     7141 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     7201 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     7261 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     7321 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     7381 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     7441 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     7501 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     7561 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     7621 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     7681 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
     7741 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     7801 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     7861 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     7921 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     7981 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     8041 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     8101 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     8161 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     8221 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     8281 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     8341 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     8401 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     8461 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     8521 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     8581 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     8641 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     8701 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     8761 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     8821 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     8881 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     8941 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     9001 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     9061 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     9121 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     9181 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     9241 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     9301 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     9361 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     9421 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     9481 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     9541 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     9601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    10201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10381 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
    10441 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
    10501 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
    10561 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
    10621 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
    10681 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
    10741 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
    10801 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
    10861 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
    10921 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
    10981 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
    11041 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
    11101 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
    11161 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
    11221 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
    11281 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
    11341 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
    11401 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
    11461 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
    11521 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
    11581 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
    11641 gctggctgg