质粒图谱
质粒序列
LOCUS Exported 10376 bp ds-DNA circular SYN 24-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10376)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 24, 2017 from Plasmid
FEATURES Location/Qualifiers
source 1..10376
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 3..27
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 113..365
/label=MAS terminator
/note="mannopine synthase terminator"
CDS complement(375..926)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=Bar
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(932..1312)
/label=MAS promoter
/note="mannopine synthase promoter (Velten et al., 1984)"
protein_bind 1364..1488
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 1513..1543
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 1597..2256
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 2598..2903
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(2944..3068)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 3070..3093
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
terminator 3147..3854
/label=OCS terminator
/note="octopine synthase terminator"
misc_feature 4098..4122
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 5422..6051
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 6480..7553
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 7619..7813
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 8157..8297
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(8483..9071)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9158..9952)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa
1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta
1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa
3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
3061 aaagtggtgg actacaaaga cgatgacgac aaatgactag gtgagtctag agagttaatt
3121 aagacccggg actagtccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc
3181 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct
3241 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat
3301 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa
3361 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc
3421 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag
3481 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca
3541 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc
3601 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct
3661 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc
3721 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc
3781 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg
3841 tcgaggctca gcaggacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
3901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
3961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
4021 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
4081 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
4141 tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
4201 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
4261 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
4321 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
4381 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
4441 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
4501 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
4561 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
4621 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
4681 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
4741 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
4801 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
4861 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
4921 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
4981 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
5041 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
5101 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
5161 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
5221 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
5281 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
5341 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
5401 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
5461 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
5521 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
5581 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
5641 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
5701 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
5761 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
5821 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
5881 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
5941 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
6001 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
6061 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
6121 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
6181 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
6241 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
6301 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
6361 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
6421 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
6481 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
6541 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
6601 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
6661 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
6721 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
6781 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
6841 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
6901 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
6961 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
7021 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
7081 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
7141 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
7201 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
7261 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
7321 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
7381 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
7441 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
7501 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
7561 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
7621 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
7681 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
7741 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
7801 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
7861 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
7921 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
7981 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
8041 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
8101 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
8161 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
8221 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
8281 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
8341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
8401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
8461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
8521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
8581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
8641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
8701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
8761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
8821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
8881 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
8941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
9001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
9061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
9121 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
9181 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
9241 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
9301 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
9361 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
9421 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
9481 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
9541 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
9601 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
9661 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
9721 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
9781 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
9841 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
9901 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
9961 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
10021 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
10081 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
10141 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
10201 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
10261 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
10321 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctgg
温馨提示：不可用于临床ZL。
