基本信息
| 原核抗性: | 氨苄青霉素Amp |
| 筛选标记: | 嘌呤霉素Puro |
| 克隆菌株: | 大肠杆菌Stbl3 |
| 培养条件: | 37℃ |
| 表达宿主: | 哺乳细胞 |
质粒简介
Ef1a_Large T-antigen_Ires_Puro是一个哺乳细胞慢病毒大T抗原表达质粒。质粒图谱
质粒序列
LOCUS Exported 11672 bp ds-DNA circular SYN 14-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..11672
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..2127
/codon_start=1
/product="large T antigen from SV40 (simian virus 40)"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
misc_feature 2159..2745
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 2746..3345
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
protein_bind 3459..3492
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 3517..3697
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 3729..3953
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3999..4427
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4441..4770
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4621..4756
/label=SV40 ori
/note="SV40 origin of replication"
promoter 4818..4865
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 4884..5258
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=Zeo
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 5388..5509
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(5960..6548)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6719..7579)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7580..7684)
/gene="bla"
/label=AmpR promoter
enhancer 7950..8329
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8331..8529
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 8547..8727
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 8774..8899
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 9392..9625
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 10152..10269
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 10405..11583
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
promoter 11600..11618
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
61 aggagtgcct gggggaatat tcctctgatg agaaaggcat atttaaaaaa atgcaaggag
121 tttcatcctg ataaaggagg agatgaagaa aaaatgaaga aaatgaatac tctgtacaag
181 aaaatggaag atggagtaaa atatgctcat caacctgact ttggaggctt ctgggatgca
241 actgagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
301 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
361 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
421 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
481 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
541 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
601 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
661 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcca
721 ttttctgtta ttgaggaaag tttgccaggt gggttaaagg agcatgattt taatccagaa
781 gaagcagagg aaactaaaca agtgtcctgg aagcttgtaa cagagtatgc aatggaaaca
841 aaatgtgatg atgtgttgtt attgcttggg atgtacttgg aatttcagta cagttttgaa
901 atgtgtttaa aatgtattaa aaaagaacag cccagccact ataagtacca tgaaaagcat
961 tatgcaaatg ctgctatatt tgctgacagc aaaaaccaaa aaaccatatg ccaacaggct
1021 gttgatactg ttttagctaa aaagcgggtt gatagcctac aattaactag agaacaaatg
1081 ttaacaaaca gatttaatga tcttttggat aggatggata taatgtttgg ttctacaggc
1141 tctgctgaca tagaagaatg gatggctgga gttgcttggc tacactgttt gttgcccaaa
1201 atggattcag tggtgtatga ctttttaaaa tgcatggtgt acaacattcc taaaaaaaga
1261 tactggctgt ttaaaggacc aattgatagt ggtaaaacta cattagcagc tgctttgctt
1321 gaattatgtg gggggaaagc tttaaatgtt aatttgccct tggacaggct gaactttgag
1381 ctaggagtag ctattgacca gtttttagta gtttttgagg atgtaaaggg cactggaggg
1441 gagtccagag atttgccttc aggtcaggga attaataacc tggacaattt aagggattat
1501 ttggatggca gtgttaaggt aaacttagaa aagaaacacc taaataaaag aactcaaata
1561 tttccccctg gaatagtcac catgaatgag tacagtgtgc ctaaaacact gcaggccaga
1621 tttgtaaaac aaatagattt taggcccaaa gattatttaa agcattgcct ggaacgcagt
1681 gagtttttgt tagaaaagag aataattcaa agtggcattg ctttgcttct tatgttaatt
1741 tggtacagac ctgtggctga gtttgctcaa agtattcaga gcagaattgt ggagtggaaa
1801 gagagattgg acaaagagtt tagtttgtca gtgtatcaaa aaatgaagtt taatgtggct
1861 atgggaattg gagttttaga ttggctaaga aacagtgatg atgatgatga agacagccag
1921 gaaaatgctg ataaaaatga agatggtggg gagaagaaca tggaagactc agggcatgaa
1981 acaggcattg attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt
2041 catgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
2101 acacctcccc ctgaacctga aacataagtt aaccgataag ggcgaattcc gccccccccc
2161 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
2221 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
2281 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
2341 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
2401 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
2461 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
2521 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
2581 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
2641 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
2701 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgac cgagtacaag
2761 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
2821 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
2881 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
2941 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
3001 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
3061 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
3121 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
3181 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
3241 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
3301 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
3361 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgaacaaaag aaaaggggac
3421 tggaagggct aattcactcc caacgaagac aagatatcat aacttcgtat agcatacatt
3481 atacgaagtt atcggctagc tggtccggac tgtactgggt ctctctggtt agaccagatc
3541 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
3601 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
3661 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
3721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
3781 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
3841 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
3901 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
3961 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
4021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
4081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
4141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
4201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
4261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
4321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
4381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
4441 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
4501 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
4561 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
4621 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
4681 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
4741 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
4801 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
4861 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
4921 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
4981 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
5041 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
5101 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
5161 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
5221 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
5281 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
5341 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
5401 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
5461 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
5521 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
5581 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
5641 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
5701 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
5761 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
5821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
5881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
5941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
6001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
6061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
6121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
6181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
6241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
6301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
6361 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
6421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
6481 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
6541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
6601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
6661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
6721 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
6781 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
6841 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
6901 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
6961 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
7021 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
7081 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
7141 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
7201 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
7261 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
7321 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
7381 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
7441 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
7501 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
7561 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
7621 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
7681 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
7741 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
7801 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
7861 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
7921 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
7981 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
8041 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
8101 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
8161 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
8221 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
8281 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
8341 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
8401 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
8461 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
8521 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
8581 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
8641 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
8701 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
8761 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
8821 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
8881 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
8941 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
9001 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
9061 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
9121 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
9181 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
9241 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
9301 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
9361 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
9421 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
9481 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
9541 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
9601 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
9661 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
9721 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
9781 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
9841 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
9901 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
9961 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
10021 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
10081 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
10141 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
10201 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
10261 caaaattttc gggtttatta cagggacagc agagatccag tttggttaat taatcgataa
10321 gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc
10381 taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg
10441 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
10501 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
10561 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
10621 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
10681 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
10741 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
10801 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
10861 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
10921 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
10981 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
11041 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
11101 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
11161 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
11221 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
11281 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
11341 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
11401 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
11461 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
11521 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
11581 tgaggaatta gcttggtact aatacgactc actataggga gacccaagct ggctaggtaa
11641 gcttgatatc gaattcgccc ttatcgtcta ga
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