基本信息
| 别名: | Gag-pol-ENY |
| 启动子: | CMV promoter |
| 复制子: | pUC ori，SV40 ori |
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 病毒系列，逆转录病毒包装载体图 |
| 质粒大小: | 12341bp |
| 原核抗性: | 氨苄青霉素Amp |
| 克隆菌株: | 大肠杆菌Stbl3 |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
pCL-Eco是一个逆转录包装质粒。质粒图谱
质粒序列
LOCUS Exported 12341 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS Untitled 10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12341)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..12341
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 283..871
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin 1107..1241
/note="SV40 ori"
/note="SV40 origin of replication"
polyA_signal 1699..1833
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(2105..2125)
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
intron 2255..2320
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
misc_feature 5033..5407
/note="pol region"
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
CDS complement(9767..10183)
/note="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
promoter complement(10495..10698)
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 10699..11078
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 11488..11592
/gene="bla"
/note="AmpR promoter"
CDS 11593..112
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
61 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
121 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
181 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
421 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
1021 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
1081 cgcgcgttgg ccgattcatt aatgcagcct ccaaaaaagc ctcctcacta cttctggaat
1141 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
1201 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt aagacccgca
1261 cccgttccca aactgcttat taaagggggt atggaggtgc tggaccttgt gacagggcca
1321 gacagtgtga cagaaataga agcagtcctg ttttgacaag ttgcctctgg aagcctctac
1381 aatgcctctc ttctttttct ccagagtaag cggaggccag gggcccccgg cctctgctta
1441 atactaaaaa aaacagctgt tgtcatagta atgattgggt ggaaacattc taggcctggg
1501 tggagaggct ttttgcttcc tcttgcaaaa ccacactgcc ctctggaggg cagttgccta
1561 gcaactaatt aaaagaggat gtcgcacggc cagctgcggt cagttagtca cttcctgctt
1621 aactgacttg acattttcta ttttaagagt cgggaggaaa attactgtgt tggaggccct
1681 ccgccatctt ctgaagctga tccagacatg ataagataca ttgatgagtt tggacaaacc
1741 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
1801 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
1861 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
1921 ggtatggctg attatgatct ctagtcaagg cactatacat caaatatttt tccataattt
1981 tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc
2041 tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt
2101 cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat
2161 catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc
2221 actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca
2281 gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg
2341 taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatcctct
2401 agctacctag ctagatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
2461 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
2521 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
2581 aaacgcgcga ggcagccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt
2641 ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc
2701 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
2761 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc ccgagtgagg
2821 ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg
2881 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
2941 aacatctgat ggttctctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
3001 ttctggagac taaataaaat cttttatttt atctatggct cgtactctat aggcttcagc
3061 tggtgatatt gttgagtcaa aactagagcc tggaccactg atatcctgtc tttaacaaat
3121 tggactaatc gattaagaat gcagggtccg aagagcaaaa tcattaggag tacaatgagg
3181 ggtcccataa tggtagatat caaggtggta aaccaagggg atctgttaaa cagtccctca
3241 aaccatcctt gagttgactc aaacagtttc tgtctctgat taagcctctc tctcaatttg
3301 gccatgctgt ctctcactag tcctgtgtgg tccgcataga agcaacattc ttcttttaga
3361 gcagcacaca gccctccttc ttttagaaat aacaagtcta ggccccttcg attctgtagg
3421 acaacttcag acagggaagt gagagacttt tctaggttag agattgattt ttcaacctcc
3481 ctgagatcat cctgtactgc ggcttggagc tgctggaatt gctgagtggc cattagagca
3541 gtagtccctg ttcctattcc agcggcaatt ccccccatgg ttagtccacc caataatagg
3601 gccagggtta acgacaccgg ttctcttttg tgtcggttgg atctctcaaa caggccgtaa
3661 acatagctgg gggaatgata ggtgactctt ggccagagtt cgacaagaac acaataatca
3721 gtggtaaggt tcagtatggt ggtggagatg catggagtaa gcccggtact acaagcccac
3781 atggtacctg taggggcaac tagataatag gaccctcgac tgcttgtctg ggtggtatta
3841 catagggcct gatgtgtttt gggaactgct cctatgcaga gtccctgtcc ggtcacttcg
3901 gacagggtca acttgtgttg ggaggccacg gagcagttgg ctggagcaga ggtatggttg
3961 gagtaggtac ccaggacggc aaccccttcg tagtaggggg gtcccgctac tagacacaac
4021 cagcactctt gggttttgtc aggactggtg aggttgaggg cttggtaggc tccgtctact
4081 aagtttagca gcctattttc cgttcccgcc ggtggaagtt gggtagggga gagaggagtc
4141 ccactgggtg gtttggtgac tgaaggcgac ttaacaggtt tgggcttgga gagtggctgt
4201 tggtctgcca gaacggggtt tggccctatt gggacgcggg gtcctagatt ttggtatctg
4261 agtcggatcc caaatgtaag ccctggatct tgtccggaga catacaaacg taagccccag
4321 taatgtcctg tggtccagga agtaacccgt ctcccggcgt ctgtaaaccg aataactaag
4381 gggttgcacc acttattatc tttgcatacc tggacagcct ggtcagaggt gagattgttg
4441 tttactgtga tgaaatccca tgatgaggag ggcttccagt aagctctacc ggttgtctca
4501 cagccccaat aggcacagta gaaggagtct ggacccccac atgacttgga ttctcggggg
4561 cggtggggcc cggggcaaac ataaaatccc tcatttgatt tatgagttgt ctggtctagc
4621 ttgagtctgt tccaggcagt gttgcaccga ggggtgaggg aggttaaagg ttcttcgcag
4681 tctctggaac agcctgggct gctgccccct gagcaacaag ggggccccgg gggagaagaa
4741 aaaggggatt gatattctag cccccaataa gatggtccat ggtgggctaa catacataaa
4801 tctggggtaa ggtcaggcca ccaggtccac agagggtggt tgccagaagt tgcccatacc
4861 gtctcccgat ctccattggt tacctcccag gtgatattat agacttgatg aggactggag
4921 ccgggcgaag cagtactgac ccctctgagc atcagaagaa ttaaggggat tagggggcct
4981 cgcgggttaa ccttattttt aaggggtttt gagagcgttg aacgcgccat gtcagtctag
5041 aggatggtcc acccccgggg tcggcagcct tcacgtgggc ggcgtgtatc caagctgcga
5101 tgccgtctac tttgagggcg gtgggggtgg tcagcaggac tgtgtaaggt cctttccagc
5161 gaggttctag gttcttagtc tggtgtcggc ggacccacac tgtgtcgccg actcggtaag
5221 ggtgaggtac caccggtcgg tccagttgtt cttggtaggc tgccgccaga ggtctccaga
5281 cttcgtgctg gactaagtag agagcctgta agtgagcttg gagagagggg ctgttagtaa
5341 ctcttgtcat gtcagggtca gggaagttta caaggggcgg gggtgcccca tataagatct
5401 catatggggt gaggccatgg gggcccggcg tgttgcgggc tcggtacagg gctaagggga
5461 gtaggagcac ccagtctcta gagccagttg caagcgttaa tttagttaaa gtctccttga
5521 tggttctatt cattctttct acctggcctg agctttgggg tctgtatgca caatgtaatt
5581 tccaatcaat ccccaacaga tcggccactg tctgactcac cttggagacg aaggcaggcc
5641 cattgtcagt tcccaatacc tgaggcatgc cgaacctggg gaagatctcc tctagtagct
5701 tcttggttac gaccttggcg gtttctttct tggttgggaa ggcttctatc cagccagaaa
5761 aggtatctat aaaaactaga agatatttat agccatacaa tccgggcttt atctcggtga
5821 aatcgatctc ccaatgagtg ccgggccgat gcccgcggac cctagttccc tgtttaacgg
5881 cagacttgct ggcgttgact tgtgcacaag ctttgcaggt ctcagtgata tttttgagtg
5941 ttcgatcccg gttcagcatg tagtagggac tgtggcttct ctctaggaga gccttcattt
6001 ttgagaagct gaggtgagtc agctgatgaa gaaagtctaa taattcaaaa gtaaactggt
6061 caggcatcac aggttttcct tggtagaccc aatacttctt tgttttatca taaatggccc
6121 ccaacttggt taggtccttt atatcagtca ctgtgtaatg aaaatgttct gaggtgtagg
6181 gtgatgaatt ttctatgagg agggtagagg tgtctggagt ctctgtgatg gctgcctttc
6241 gggccgcttg gtcagccatc cggttgcctc tagcctcggc gctgtgtccc ttttgatgtc
6301 ctggacaatg gattatgcta agtcttttgg gcagaaagag ggcttttagt agggccaaga
6361 tctcgtcttt atttttgatc tctttgcctt ctgatgtgag caacccacgc cttctgtata
6421 tttctccatg gatatgggca gtagcaaaag cataacggct atcagtataa acatttagct
6481 tcttaccttc tgccatcttt agggcctggg tgagtgctat cagttcagcc cgctgagcgg
6541 atgtcccggc tggcagggct ttagcccaga ttacctcggt ctcggtggtc accgcagctc
6601 ccgccttacg ctgtccctct tgtaagagac tgcttccatc cgtgtaccag gtgtggtcgg
6661 cgtctgggag cggctggtcc gttaggtcgg gtcgggttcc gtgggcttcg gccaggatat
6721 caaggcagtt gtgttgcagc ccttcctcag gcagtgggag cagcgtagcc gggttcaggg
6781 ctaccaccgg tccgaactgg acccggtccg tgtccaaaag caaggcctga tagtgagtca
6841 tccgggcgtt ggaaagccag cggtcggggg gttgtttgac tagtgcctct actgcatggg
6901 gggccagaat gactagtggc tgtcccatgg ttagcttgcc tgcatccttt gtcagtacgg
6961 caatggctgc taccatccgt aggcaagggg gccacccagc tgctactggg tctagctttt
7021 tggacaggta ggccaccggc cgacgccaag gtcccagttt ttgcgttagg acacctttgg
7081 cgtagccctg cttctcgtcg acaaagagtt caaagggctt agtcaaatct ggcaacccca
7141 gggctggggc agttagaaga gcttgcttga tttcttgata ggccttttgt tggtctgggc
7201 cccaattaaa cagagtcccc gttttggtga gagggtacaa gggggctgcc atttctgcaa
7261 acccagggat ccagaggcga cagaagcctg ccgtccctag gaactccctt agttgtcgag
7321 gggtcttcgg agtaggctgc cccatcacag tctcttttct ggcctcagtc agccatctct
7381 gaccctcttt tagaagatac cccagatact tgacctgttt ctggcaaatt tgggctttct
7441 tggccgaggc ccgatacccg aggttcccta gggtttgtaa cagggcccga gtaccttgtt
7501 ggcagtctag ctcagaagtg gcggccagca gtaagtcatc cacgtactgt agcaggatca
7561 agtctgggtg ctggatccgg aagtctgcta ggtctctgtg cagtgcctca tcaaacaggg
7621 tgggactgtt tttgaaaccc tgtgggagtc tggtccaggt caattgtcct gagattccca
7681 tctctggatc tctccactca aaggcgaaga gaggctgact ggtggggtgg agtctcaggc
7741 agaaaaaggc atcctttaaa tcaagcacag tgtaccactg gtgggacggt gggagcccgc
7801 tcaagaggtt gtaagggttg ggcacggtgg ggtggatgtc ttccacccgc ttgttgactt
7861 ctctcagatc ctggacaggc ctataatcat tagtccctgg tttcttaacg ggtagcaggg
7921 gcgtgttcca gggggactgg cagggtacca gtattccctg gtccaacagt ctctgtatgt
7981 ggggcttgat ccccagtctg gcttcttgtg acatggggta ttgttttatg gacacggggg
8041 tagaggttgc tttcagaggt atgatcagag gagcttggcg aactgccagt cccatgcccc
8101 cggtttccgc ccaggcctga ggaaaatcag acagccatgt ggaccctaga gaaacatctg
8161 gctcttttga ggtctcatgt agccgatact catcttctat atttagggtc aacacttgca
8221 ggggctgccc cattggtccc ataacctgag ctcctgatcc ctcaaagtgg atttgggctt
8281 ttagtttagt cagcaaatct cttcctaaca gaggataggg acagtctggt acatggagga
8341 aagagtgggt gaccttaccg gtagctagat gtactttgcg atccgtggtc cagcgatacc
8401 gctttcctcc agtagcccct tggacccagg cagacttatc acttaggggt ccaggatttt
8461 gggtcagcac ggagtgttgg gccccagtat ctaccaggaa ggtgacgggt tgccccccga
8521 ctttgagggt tatcctgggt tcaggggggg gctcctgacc ctgacctccc tagtcatcta
8581 gggtcaggag ggaggtctgg ggtcttggtc cccgaggtcc tcgtggtttc ttgggacaat
8641 ctttagccca gtgccccttt tctttgcagt aggcacactg gtcgcgatcg agttgggacc
8701 tccttcgttc tcctccctgt ctatcctgtt tctgtccact aacgacagtg gccaatagct
8761 tgctcatctc tctatgtctc ctacgatctc tttctttctc tttctgctca tcctctgtcc
8821 tacggcgttc ttctttttcc tctgtttctc tcctgatacg ttcctctctt tcttccgggg
8881 tttctcgttt attaaagatc ttttctgcct ctctaaccaa atctccaagc gtcttgtttt
8941 ttaaatcttc taacctctct aactttctcc caatgtctgg ggcagactgc caaatgaaag
9001 acatagacac attagtttct tgccctgggt cctcagggtc ataaggagtg tacctgcgat
9061 aggcttcctt aagtctctct aggaaggccg agggagactc attgggccct tgtgttattc
9121 cttttacctt ggccaaattg gtggggcttc tgcccgcgtt ttggagaccc gctaggagca
9181 actggcgata gtggactagg tggttcctac ctgcctgggt ggtgtaatcc cagtctgggc
9241 gctcgagggg aaaagcggca tcgacttcat tgggcagttg agtggggcgc ccatcatcgc
9301 cccgcaccgc ctttctagcc tctaagagca cccgttgttt ttcttctccg gtcagcagag
9361 tccccaacag ctgctgacag tcgtcccagg tgggctgatg ggtgatgagg acagactcga
9421 tcagagctgt cagtttacct ggatcttcag aaaaagaagg gttattattt ttccagttgt
9481 aaaggtcaga agaggagaac ggccagtatt gaagctgtcc gtttcctcct gcgcggaggg
9541 ggaatgcctg cgaggtagtg gagtcggcca cagggggctc ccgtctccca cgtaggcgag
9601 atgccattgg ggaggggtcc ggtgcctctc ccgcaggggt cgcttctcca ccatttccgt
9661 ccctgtcgga agggggtggt cttgggtccc tataaggcgg ggggtcttct gtaagtaggt
9721 cgatgagcgg ccccccactg tcagaaagaa cttgaggttt aggtttggcg cctagagaag
9781 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
9841 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
9901 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
9961 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
10021 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
10081 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
10141 agtgacctaa ggtcaaactt aagggagtgg taacagtctg gcccatattt tcagacaaat
10201 acagaaacac agtcagacag agacaacaca gaacgatgcc caagcttggc cagcttacct
10261 cccggtggtg ggtcggtggt ccctgggcag gggtctcccg atcccggacg agcccccaaa
10321 tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca aggaacagcg
10381 agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac
10441 tcagtcaatc ggaggactgg cgcgccgagt gaggggttgt gggctctttt attgagctct
10501 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
10561 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
10621 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
10681 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
10741 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
10801 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
10861 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
10921 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
10981 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
11041 aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgctggcg taatagcgaa
11101 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
11161 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
11221 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
11281 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
11341 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
11401 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
11461 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
11521 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
11581 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
11641 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
11701 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
11761 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
11821 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
11881 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
11941 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
12001 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
12061 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
12121 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
12181 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
12241 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
12301 gagcgtgggt ctcgcggtat cattgcagca ctggggccag a
温馨提示：不可用于临床ZL。
