基本信息
| 复制子: | pUC ori，f1 ori |
| 质粒分类: | 腺相关病毒包装质粒 |
| 质粒大小: | 11635bp |
| 原核抗性: | 氨苄青霉素Amp |
| 克隆菌株: | 大肠杆菌Stbl3 |
| 培养条件: | 37℃，有氧，LB |
| 表达宿主: | 哺乳细胞 |
| 5'测序引物: | 根据序列设计引物 |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
pHelper质粒是一个腺相关病毒骨架包装质粒，可与pAAV-MCS和pAAV-RC2共同包装成腺相关病毒。
This 11.6 KB vector carries the adenovirus genes (E2A E4 and VA RNAs required inducing the lytic) for phase of AAV. This vector is cotransfected into the packaging cell line to produce recombinant replication-deficient AAV virions., it is related to packaging adenovirus vector with ampicillin resistance.质粒图谱
质粒序列
LOCUS Exported 11635 bp ds-DNA circular SYN 15-AUG-2018
DEFINITION synthetic circular DNA
KEYWORDS pHelper
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11635)
AUTHORS Triple Threat
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..11635
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature complement(1..5336)
/note="adeno E2A"
misc_feature 5337..8537
/note="adeno E4"
misc_feature complement(8538..9280)
/note="adeno VA"
rep_origin complement(9423..10011)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10182..11042)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(11043..11147)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(11173..11627)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ggtacccaac tccatgctta acagtcccca ggtacagccc accctgcgtc gcaaccagga
61 acagctctac agcttcctgg agcgccactc gccctacttc cgcagccaca gtgcgcagat
121 taggagcgcc acttcttttt gtcacttgaa aaacatgtaa aaataatgta ctaggagaca
181 ctttcaataa aggcaaatgt ttttatttgt acactctcgg gtgattattt accccccacc
241 cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct atgcgccact
301 ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg cacaaccatc
361 cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa cgcgtttagc
421 aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc gcgcgagttg
481 cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac gctggccagc
541 acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag ggcgaacgga
601 gtcaactttg gtagctgcct tcccaaaaag ggtgcatgcc caggctttga gttgcactcg
661 caccgtagtg gcatcagaag gtgaccgtgc ccggtctggg cgttaggata cagcgcctgc
721 atgaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga gaagaacatg
781 ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcatgcac gcagcacctt
841 gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac gatcttggcc
901 ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc catttcaatc
961 acgtgctcct tatttatcat aatgctcccg tgtagacact taagctcgcc ttcgatctca
1021 gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt ggtgcttgta ggttacctct
1081 gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa ggtcttgttg
1141 ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttta gccaggtctt gcatacggcc
1201 gccagagctt ccacttggtc aggcagtagc ttgaagtttg cctttagatc gttatccacg
1261 tggtacttgt ccatcaacgc gcgcgcagcc tccatgccct tctcccacgc agacacgatc
1321 ggcaggctca gcgggtttat caccgtgctt tcactttccg cttcactgga ctcttccttt
1381 tcctcttgcg tccgcatacc ccgcgccact gggtcgtctt cattcagccg ccgcaccgtg
1441 cgcttacctc ccttgccgtg cttgattagc accggtgggt tgctgaaacc caccatttgt
1501 agcgccacat cttctctttc ttcctcgctg tccacgatca cctctgggga tggcgggcgc
1561 tcgggcttgg gagaggggcg cttctttttc tttttggacg caatggccaa atccgccgtc
1621 gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg catcttgtga cgagtcttct
1681 tcgtcctcgg actcgagacg ccgcctcagc cgcttttttg ggggcgcgcg gggaggcggc
1741 ggcgacggcg acggggacga cacgtcctcc atggttggtg gacgtcgcgc cgcaccgcgt
1801 ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc cttctcctat
1861 aggcagaaaa agatcatgga gtcagtcgag aaggaggaca gcctaaccgc cccctttgag
1921 ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc cgtcgaggca
1981 cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt aagcgaagac
2041 gacgaggatc gctcagtacc aacagaggat aaaaagcaag accaggacga cgcagaggca
2101 aacgaggaac aagtcgggcg gggggaccaa aggcatggcg actacctaga tgtgggagac
2161 gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc gttgcaagag
2221 cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg ccacctgttc
2281 tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa cccgcgcctc
2341 aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat ctttttccaa
2401 aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa gcagctggcc
2461 ttgcggcagg gcgctgtcat acctgatatc gcctcgctcg acgaagtgcc aaaaatcttt
2521 gagggtcttg gacgcgacga gaaacgcgcg gcaaacgctc tgcaacaaga aaacagcgaa
2581 aatgaaagtc actgtggagt gctggtggaa cttgagggtg acaacgcgcg cctagccgtg
2641 ctgaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct accccccaag
2701 gttatgagca cagtcatgag cgagctgatc gtgcgccgtg cacgacccct ggagagggat
2761 gcaaacttgc aagaacaaac cgaggagggc ctacccgcag ttggcgatga gcagctggcg
2821 cgctggcttg agacgcgcga gcctgccgac ttggaggagc gacgcaagct aatgatggcc
2881 gcagtgcttg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga cccggagatg
2941 cagcgcaagc tagaggaaac gttgcactac acctttcgcc agggctacgt gcgccaggcc
3001 tgcaaaattt ccaacgtgga gctctgcaac ctggtctcct accttggaat tttgcacgaa
3061 aaccgcctcg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg ccgcgactac
3121 gtccgcgact gcgtttactt atttctgtgc tacacctggc aaacggccat gggcgtgtgg
3181 cagcaatgcc tggaggagcg caacctaaag gagctgcaga agctgctaaa gcaaaacttg
3241 aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc ggacattatc
3301 ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac cagtcaaagc
3361 atgttgcaaa actttaggaa ctttatccta gagcgttcag gaattctgcc cgccacctgc
3421 tgtgcgcttc ctagcgactt tgtgcccatt aagtaccgtg aatgccctcc gccgctttgg
3481 ggtcactgct accttctgca gctagccaac taccttgcct accactccga catcatggaa
3541 gacgtgagcg gtgacggcct actggagtgt cactgtcgct gcaacctatg caccccgcac
3601 cgctccctgg tctgcaattc gcaactgctt agcgaaagtc aaattatcgg tacctttgag
3661 ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact cactccgggg
3721 ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc ccacgagatt
3781 aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg cgtcattacc
3841 cagggccaca tccttggcca attgcaagcc atcaacaaag cccgccaaga gtttctgcta
3901 cgaaagggac ggggggttta cctggacccc cagtccggcg aggagctcaa cccaatcccc
3961 ccgccgccgc agccctatca gcagccgcgg gcccttgctt cccaggatgg cacccaaaaa
4021 gaagctgcag ctgccgccgc cgccacccac ggacgaggag gaatactggg acagtcaggc
4081 agaggaggtt ttggacgagg aggaggagat gatggaagac tgggacagcc tagacgaagc
4141 ttccgaggcc gaagaggtgt cagacgaaac accgtcaccc tcggtcgcat tcccctcgcc
4201 ggcgccccag aaattggcaa ccgttcccag catcgctaca acctccgctc ctcaggcgcc
4261 gccggcactg cctgttcgcc gacccaaccg tagatgggac accactggaa ccagggccgg
4321 taagtctaag cagccgccgc cgttagccca agagcaacaa cagcgccaag gctaccgctc
4381 gtggcgcggg cacaagaacg ccatagttgc ttgcttgcaa gactgtgggg gcaacatctc
4441 cttcgcccgc cgctttcttc tctaccatca cggcgtggcc ttcccccgta acatcctgca
4501 ttactaccgt catctctaca gcccctactg caccggcggc agcggcagcg gcagcaacag
4561 cagcggtcac acagaagcaa aggcgaccgg atagcaagac tctgacaaag cccaagaaat
4621 ccacagcggc ggcagcagca ggaggaggag cgctgcgtct ggcgcccaac gaacccgtat
4681 cgacccgcga gcttagaaat aggatttttc ccactctgta tgctatattt caacaaagca
4741 ggggccaaga acaagagctg aaaataaaaa acaggtctct gcgctccctc acccgcagct
4801 gcctgtatca caaaagcgaa gatcagcttc ggcgcacgct ggaagacgcg gaggctctct
4861 tcagcaaata ctgcgcgctg actcttaagg actagtttcg cgccctttct caaatttaag
4921 cgcgaaaact acgtcatctc cagcggccac acccggcgcc agcacctgtc gtcagcgcca
4981 ttatgagcaa ggaaattccc acgccctaca tgtggagtta ccagccacaa atgggacttg
5041 cggctggagc tgcccaagac tactcaaccc gaataaacta catgagcgcg ggaccccaca
5101 tgatatcccg ggtcaacgga atccgcgccc accgaaaccg aattctcctc gaacaggcgg
5161 ctattaccac cacacctcgt aataacctta atccccgtag ttggcccgct gccctggtgt
5221 accaggaaag tcccgctccc accactgtgg tacttcccag agacgcccag gccgaagttc
5281 agatgactaa ctcaggggcg cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg
5341 ggcgttttag ggcggagtaa cttgcatgta ttgggaattg tagttttttt aaaatgggaa
5401 gtgacgtatc gtgggaaaac ggaagtgaag atttgaggaa gttgtgggtt ttttggcttt
5461 cgtttctggg cgtaggttcg cgtgcggttt tctgggtgtt ttttgtggac tttaaccgtt
5521 acgtcatttt ttagtcctat atatactcgc tctgtacttg gcccttttta cactgtgact
5581 gattgagctg gtgccgtgtc gagtggtgtt ttttaatagg tttttttact ggtaaggctg
5641 actgttatgg ctgccgctgt ggaagcgctg tatgttgttc tggagcggga gggtgctatt
5701 ttgcctaggc aggagggttt ttcaggtgtt tatgtgtttt tctctcctat taattttgtt
5761 atacctccta tgggggctgt aatgttgtct ctacgcctgc gggtatgtat tcccccgggc
5821 tatttcggtc gctttttagc actgaccgat gttaaccaac ctgatgtgtt taccgagtct
5881 tacattatga ctccggacat gaccgaggaa ctgtcggtgg tgctttttaa tcacggtgac
5941 cagttttttt acggtcacgc cggcatggcc gtagtccgtc ttatgcttat aagggttgtt
6001 tttcctgttg taagacaggc ttctaatgtt taaatgtttt tttttttgtt attttatttt
6061 gtgtttaatg caggaacccg cagacatgtt tgagagaaaa atggtgtctt tttctgtggt
6121 ggttccggaa cttacctgcc tttatctgca tgagcatgac tacgatgtgc ttgctttttt
6181 gcgcgaggct ttgcctgatt ttttgagcag caccttgcat tttatatcgc cgcccatgca
6241 acaagcttac ataggggcta cgctggttag catagctccg agtatgcgtg tcataatcag
6301 tgtgggttct tttgtcatgg ttcctggcgg ggaagtggcc gcgctggtcc gtgcagacct
6361 gcacgattat gttcagctgg ccctgcgaag ggacctacgg gatcgcggta tttttgttaa
6421 tgttccgctt ttgaatctta tacaggtctg tgaggaacct gaatttttgc aatcatgatt
6481 cgctgcttga ggctgaaggt ggagggcgct ctggagcaga tttttacaat ggccggactt
6541 aatattcggg atttgcttag agacatattg ataaggtggc gagatgaaaa ttatttgggc
6601 atggttgaag gtgctggaat gtttatagag gagattcacc ctgaagggtt tagcctttac
6661 gtccacttgg acgtgagggc agtttgcctt ttggaagcca ttgtgcaaca tcttacaaat
6721 gccattatct gttctttggc tgtagagttt gaccacgcca ccggagggga gcgcgttcac
6781 ttaatagatc ttcattttga ggttttggat aatcttttgg aataaaaaaa aaaaaacatg
6841 gttcttccag ctcttcccgc tcctcccgtg tgtgactcgc agaacgaatg tgtaggttgg
6901 ctgggtgtgg cttattctgc ggtggtggat gttatcaggg cagcggcgca tgaaggagtt
6961 tacatagaac ccgaagccag ggggcgcctg gatgctttga gagagtggat atactacaac
7021 tactacacag agcgagctaa gcgacgagac cggagacgca gatctgtttg tcacgcccgc
7081 acctggtttt gcttcaggaa atatgactac gtccggcgtt ccatttggca tgacactacg
7141 accaacacga tctcggttgt ctcggcgcac tccgtacagt agggatcgcc tacctccttt
7201 tgagacagag acccgcgcta ccatactgga ggatcatccg ctgctgcccg aatgtaacac
7261 tttgacaatg cacaacgtga gttacgtgcg aggtcttccc tgcagtgtgg gatttacgct
7321 gattcaggaa tgggttgttc cctgggatat ggttctgacg cgggaggagc ttgtaatcct
7381 gaggaagtgt atgcacgtgt gcctgtgttg tgccaacatt gatatcatga cgagcatgat
7441 gatccatggt tacgagtcct gggctctcca ctgtcattgt tccagtcccg gttccctgca
7501 gtgcatagcc ggcgggcagg ttttggccag ctggtttagg atggtggtgg atggcgccat
7561 gtttaatcag aggtttatat ggtaccggga ggtggtgaat tacaacatgc caaaagaggt
7621 aatgtttatg tccagcgtgt ttatgagggg tcgccactta atctacctgc gcttgtggta
7681 tgatggccac gtgggttctg tggtccccgc catgagcttt ggatacagcg ccttgcactg
7741 tgggattttg aacaatattg tggtgctgtg ctgcagttac tgtgctgatt taagtgagat
7801 cagggtgcgc tgctgtgccc ggaggacaag gcgtctcatg ctgcgggcgg tgcgaatcat
7861 cgctgaggag accactgcca tgttgtattc ctgcaggacg gagcggcggc ggcagcagtt
7921 tattcgcgcg ctgctgcagc accaccgccc tatcctgatg cacgattatg actctacccc
7981 catgtaggcg tggacttccc cttcgccgcc cgttgagcaa ccgcaagttg gacagcagcc
8041 tgtggctcag cagctggaca gcgacatgaa cttaagcgag ctgcccgggg agtttattaa
8101 tatcactgat gagcgtttgg ctcgacagga aaccgtgtgg aatataacac ctaagaatat
8161 gtctgttacc catgatatga tgctttttaa ggccagccgg ggagaaagga ctgtgtactc
8221 tgtgtgttgg gagggaggtg gcaggttgaa tactagggtt ctgtgagttt gattaaggta
8281 cggtgatcaa tataagctat gtggtggtgg ggctatacta ctgaatgaaa aatgacttga
8341 aattttctgc aattgaaaaa taaacacgtt gaaacataac atgcaacagg ttcacgattc
8401 tttattcctg ggcaatgtag gagaaggtgt aagagttggt agcaaaagtt tcagtggtgt
8461 attttccact ttcccaggac catgtaaaag acatagagta agtgcttacc tcgctagttt
8521 ctgtggattc actagaatcg atgtaggatg ttgcccctcc tgacgcggta ggagaagggg
8581 agggtgccct gcatgtctgc cgctgctctt gctcttgccg ctgctgagga ggggggcgca
8641 tctgccgcag caccggatgc atctgggaaa agcaaaaaag gggctcgtcc ctgtttccgg
8701 aggaatttgc aagcggggtc ttgcatgacg gggaggcaaa cccccgttcg ccgcagtccg
8761 gccggcccga gactcgaacc gggggtcctg cgactcaacc cttggaaaat aaccctccgg
8821 ctacagggag cgagccactt aatgctttcg ctttccagcc taaccgctta cgccgcgcgc
8881 ggccagtggc caaaaaagct agcgcagcag ccgccgcgcc tggaaggaag ccaaaaggag
8941 cgctcccccg ttgtctgacg tcgcacacct gggttcgaca cgcgggcggt aaccgcatgg
9001 atcacggcgg acggccggat ccggggttcg aaccccggtc gtccgccatg atacccttgc
9061 gaatttatcc accagaccac ggaagagtgc ccgcttacag gctctccttt tgcacggtct
9121 agagcgtcaa cgactgcgca cgcctcaccg gccagagcgt cccgaccatg gagcactttt
9181 tgccgctgcg caacatctgg aaccgcgtcc gcgactttcc gcgcgcctcc accaccgccg
9241 ccggcatcac ctggatgtcc aggtacatct acggattacg tcgacgttta aaccatatga
9301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
9361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
9421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
9481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
9541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
9601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
9661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
9721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
9781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
9841 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
9901 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
9961 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
10021 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
10081 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
10141 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
10201 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
10261 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
10321 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
10381 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
10441 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
10501 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
10561 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
10621 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
10681 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
10741 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
10801 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
10861 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
10921 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
10981 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
11041 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
11101 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
11161 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
11221 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
11281 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
11341 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
11401 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
11461 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
11521 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
11581 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgatg gatcc
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