基本信息
| 启动子: | CMV |
| 复制子: | pUC ori |
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 病毒系列，腺病毒 |
| 质粒大小: | 8175bp |
| 原核抗性: | Kan |
| 筛选标记: | Neo |
| 克隆菌株: | Stbl3 |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | pEGFP-N-3:CGTCGCCGTCCAGCTCGACCAG |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
pAdTrack载体用来转基因腺病毒细胞表达，载体拥有一个EGFP绿色荧光蛋白跟踪基因表达结果，和pAdeasy1载体共同使用时能够插入5.9kb，pAdeasy2载体共同使用时能够插入8.6kb片段。具体使用方法可以参考pAdeasy载体系统。
质粒图谱
质粒序列
LOCUS Exported 8175 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8175)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..8175
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 363..484
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(623..1339)
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
promoter complement(1384..1587)
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 1588..1891
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
rep_origin complement(5502..6090)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 6903..7697
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
gap 8112..8175
/estimated_length="64"
ORIGIN
1 nnnttaatta annntccctt ccagctctct gccccttttg gattgaagcc aatatgataa
61 tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag
121 tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa
181 aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta
241 ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa
301 actgaataag aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaannncgc
361 gttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
421 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
481 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
541 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
601 atccggtgga tctgagtccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg
661 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
721 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
781 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
841 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
901 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
961 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
1021 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
1081 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
1141 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
1201 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
1261 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
1321 cctcgccctt gctcaccatg gtggcgaccg gtagcgctag cggatctgac ggttcactaa
1381 accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
1441 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
1501 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
1561 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
1621 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
1681 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
1741 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
1801 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
1861 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
1921 tgaccccgta attgattact attannncta gcagatctgg taccgtcgac gcggccgcga
1981 tatcctcgag aagctttcta gagnnntaag ggtgggaaag aatatataag gtgggggtct
2041 tatgtagttt tgtatctgtt ttgcagcagc cgccgccgcc atgagcacca actcgtttga
2101 tggaagcatt gtgagctcat atttgacaac gcgcatgccc ccatgggccg gggtgcgtca
2161 gaatgtgatg ggctccagca ttgatggtcg ccccgtcctg cccgcaaact ctactacctt
2221 gacctacgag accgtgtctg gaacgccgtt ggagactgca gcctccgccg ccgcttcagc
2281 cgctgcagcc accgcccgcg ggattgtgac tgactttgct ttcctgagcc cgcttgcaag
2341 cagtgcagct tcccgttcat ccgcccgcga tgacaagttg acggctcttt tggcacaatt
2401 ggattctttg acccgggaac ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca
2461 ggtttctgcc ctgaaggctt cctcccctcc caatgcggtt taaaacataa ataaaaaacc
2521 agactctgtt tggatttgga tcaagcaagt gtcttgctgt ctttatttag gggttttgcg
2581 cgcgcggtag gcccgggacc agcggtctcg gtcgttgagg gtcctgtgta ttttttccag
2641 gacgtggtaa aggtgactct ggatgttcag atacatgggc ataagcccgt ctctggggtg
2701 gaggtagcac cactgcagag cttcatgctg cggggtggtg ttgtagatga tccagtcgta
2761 gcaggagcgc tgggcgtggt gcctaaaaat gtctttcagt agcaagctga ttgccagggg
2821 caggcccttg gtgtaagtgt ttacaaagcg gttaagctgg gatgggtgca tacgtgggga
2881 tatgagatgc atcttggact gtatttttag gttggctatg ttcccagcca tatccctccg
2941 gggattcatg ttgtgcagaa ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc
3001 atgtagctta gaaggaaatg cgtggaagaa cttggagacg cccttgtgac ctccaagatt
3061 ttccatgcat tcgtccataa tgatggcaat gggcccacgg gcggcggcct gggcgaagat
3121 atttctggga tcactaacgt catagttgtg ttccaggatg agatcgtcat aggccatttt
3181 tacaaagcgc gggcggaggg tgccagactg cggtataatg gttccatccg gcccaggggc
3241 gtagttaccc tcacagattt gcatttccca cgctttgagt tcagatgggg ggatcatgtc
3301 tacctgcggg gcgatgaaga aaacggtttc cggggtaggg gagatcagct gggaagaaag
3361 caggttcctg agcagctgcg acttaccgca gccggtgggc ccgtaaatca cacctattac
3421 cgggtgcaac tggtagttaa gagagctgca gctgccgtca tccctgagca ggggggccac
3481 ttcgttaagc atgtccctga ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc
3541 gccgcccagc gatagcagtt cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc
3601 cgccgtaggc atgcttttga gcgtttgacc aagcagttcc aggcggtccc acagctcggt
3661 cacctgctct acggcatctc gatccagcat atctcctcgt ttcgcgggtt ggggcggctt
3721 tcgctgtacg gcagtagtcg gtgctcgtcc agacgggcca gggtcatgtc tttccacggg
3781 cgcagggtcc tcgtcagcgt agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg
3841 ctggccaggg tgcgcttgag gctggtcctg ctggtgctga agcgctgccg gtcttcgccc
3901 tgcgcgtcgg ccaggtagca tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg
3961 cccttggcgc gcagcttgcc cttggaggag gcgccgcacg aggggcagtg cagacttttg
4021 agggcgtaga gcttgggcgc gagaaatacc gattccgggg agtaggcatc cgcgccgcag
4081 gccccgcaga cggtctcgca ttccacgagc caggtgagct ctggccgttc ggggtcaaaa
4141 accaggtttc ccccatgctt tttgatgcgt ttcttacctc tggtttccat gagccggtgt
4201 ccacgctcgg tgacgaaaag gctgtccgtg tccccgtata cagactnnng tttaaacgaa
4261 ttcnnntata aaatgcaagg tgctgctcaa aaaatcaggc aaagcctcgc gcaaaaaaga
4321 aagcacatcg tagtcatgct catgcagata aaggcaggta agctccggaa ccaccacaga
4381 aaaagacacc atttttctct caaacatgtc tgcgggtttc tgcataaaca caaaataaaa
4441 taacaaaaaa acatttaaac attagaagcc tgtcttacaa caggaaaaac aacccttata
4501 agcataagac ggactacggc catgccggcg tgaccgtaaa aaaactggtc accgtgatta
4561 aaaagcacca ccgacagctc ctcggtcatg tccggagtca taatgtaaga ctcggtaaac
4621 acatcaggtt gattcatcgg tcagtgctaa aaagcgaccg aaatagcccg ggggaataca
4681 tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa aattaatagg
4741 agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg
4801 ctccagaaca acatacagcg cttcacagcg gcagcctaac agtcagcctt accagtaaaa
4861 aagaaaacct attaaaaaaa caccactcga cacggcacca gctcaatcag tcacagtgta
4921 aaaaagggcc aagtgcagag cgagtatata taggactaaa aaatgacgta acggttaaag
4981 tccacaaaaa acacccagaa aaccgcacgc gaacctacgc ccagaaacga aagccaaaaa
5041 acccacaact tcctcaaatc gtcacttccg ttttcccacg ttacgtaact tcccatttta
5101 agaaaactac aattcccaac acatacaagt tactccgccc taaaacctac gtcacccgcc
5161 ccgttcccac gccccgcgcc acgtcacaaa ctccaccccc tcattatcat attggcttca
5221 atccaaaata aggtatatta ttgatgatnn nttaattaag gatccnnncg gtgtgaaata
5281 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
5341 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
5401 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
5461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
5521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
5581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
5641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
5761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
5821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
5881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
5941 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
6001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
6061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
6121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
6181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
6241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
6301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
6361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
6421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
6481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
6541 ccagttaata gtttgcgcaa cgttgttgnn nnnnaaaaag gatcttcacc tagatccttt
6601 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
6661 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
6721 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
6781 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc
6841 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
6901 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
6961 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
7021 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
7081 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
7141 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
7201 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
7261 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
7321 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
7381 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
7441 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
7501 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
7561 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
7621 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
7681 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa
7741 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt
7801 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
7861 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
7921 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga
7981 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
8041 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc
8101 ttaatgcgcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
8161 nnnnnnnnnn nnnnn
温馨提示：不可用于临床ZL。
