基本信息
| 启动子: | TK |
| 复制子: | pUC ori |
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 哺乳细胞，信号通路报告载体 |
| 原核抗性: | 氨苄青霉素Amp |
| 克隆菌株: | 大肠杆菌DH5α |
| 培养条件: | 37℃，有氧，LB |
| 表达宿主: | 哺乳细胞 |
| 5'测序引物: | 根据序列设计引物 |
| 3'测序引物: | 根据序列设计引物 |
质粒图谱
质粒序列
LOCUS Exported 5529 bp ds-DNA circular SYN 24-APR-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5529
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 590..652
/note="Mini-TK promoter"
/note="minimal herpes simplex virus (HSV) thymidine kinase
promoter"
CDS 681..2333
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/note="luciferase"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 2492..2557
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
CDS 2687..2707
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
polyA_signal 3132..3266
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3710..4298)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4469..5329)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5330..5434)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa agggggtaag gccaaagggg
421 gtaaggccaa agggggcccc ctttggcctt accccctttg gccttacccc ctttggcctt
481 acctgcatgc ctgcaggtcg actctagagg atccggcccc gcccagcgtc ttgtcattgg
541 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
601 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
661 acagcgtgcc gcagatctcc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
721 tctatcctct agaggatgga accgctggag agcaactgca taaggctatg aagagatacg
781 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg
841 cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
901 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
961 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
1021 tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc
1081 aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt
1141 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
1201 ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac
1261 tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa
1321 ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
1381 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
1441 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt
1501 tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat
1561 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
1621 ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc
1681 cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg
1741 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
1801 tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag
1861 gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
1921 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
1981 tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat
2041 tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg
2101 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
2161 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
2221 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
2281 aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg taaaatgtaa
2341 ctgtattcag cgatgacgaa attcttagct attgtaatga attcgagctc caccgcggtg
2401 gcggccgctc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
2461 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
2521 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
2581 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
2641 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
2701 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
2761 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
2821 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
2881 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
2941 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
3001 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
3061 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
3121 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
3181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
3241 tcaatgtatc ttatcatgtc tggatctcga gggggggccc ggtaccgagc tcgaattcgt
3301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
3361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
3421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
3481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
3541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
3601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
3661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
3721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
3781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
3841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
3901 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
3961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
4141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
4201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
4261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
4321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
4381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
4441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
4501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
4561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
4621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
4681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
4741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
4801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
4861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
4921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
4981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
5101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
5341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
5401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
5461 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
5521 cctttcgtc
温馨提示：不可用于临床ZL。
