基本信息
| 启动子: | Lac/lac |
| 复制子: | ColE1 ori |
| 质粒分类: | 大肠杆菌载体；克隆质粒 |
| 质粒大小: | 2710bp |
| 质粒标签: | LacZ |
| 原核抗性: | 氨苄青霉素Amp |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧，LB |
| 5'测序引物: | M13R:CAGGAAACAGCTATGACC |
| 3'测序引物: | M13F:TGTAAAACGACGGCCAGT |
| 备注: | 蓝白斑筛选，单一的EcoRV酶切位点 |
质粒简介
质粒图谱
质粒序列
LOCUS Exported File 2710 bp ds-DNA circular SYN 02-2-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2710)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-2-2 from MLCC
FEATURES Location/Qualifiers
source 1..2710
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 379..395
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(489..505)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 513..529
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(537..567)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 582..603
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(891..1479)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1650..2510)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2511..2615)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggagatcggt acttcgcgaa
421 tgcgtcgaga tatcggatgc cgggaccgac gagtgcagag gcgtgcaagc gagcttggcg
481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
1021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
1081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
1141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
1201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
1261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
1321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
1381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
1441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
1501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
1561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
1621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
1681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
1741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
1801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
1861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
1921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
1981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
2041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
2101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
2161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
2221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
2281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
2341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
2401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
2461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
2521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
2581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
2641 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
2701 ccctttcgtc
温馨提示：不可用于临床ZL。
