基本信息
| 别名: | NM_174936.3 |
| 原核抗性: | 氨苄青霉素Amp |
| 筛选标记: | 嘌呤霉素Puro |
| 克隆菌株: | 大肠杆菌Stbl3 |
| 培养条件: | 37℃ |
质粒简介
pLVML-ECMV-PCSK9(NM_174936.3) 是一个人源ORF基因哺乳细胞表达质粒。质粒图谱
质粒序列
LOCUS Exported 10203 bp ds-DNA circular SYN 20-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..10203
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 2809..4887
/label=PCSK9
misc_feature 4910..5483
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 5516..6115
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
misc_feature 6129..6717
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 6924..7557
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
rep_origin complement(8087..8675)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8842..9702)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(9703..9807)
/gene="bla"
/label=AmpR promoter
polyA_signal 9855..9989
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcat gggcaccgtc
2821 agctccaggc ggtcctggtg gccgctgcca ctgctgctgc tgctgctgct gctcctgggt
2881 cccgcgggcg cccgtgcgca ggaggacgag gacggcgact acgaggagct ggtgctagcc
2941 ttgcgttccg aggaggacgg cctggccgaa gcacccgagc acggaaccac agccaccttc
3001 caccgctgcg ccaaggatcc gtggaggttg cctggcacct acgtggtggt gctgaaggag
3061 gagacccacc tctcgcagtc agagcgcact gcccgccgcc tgcaggccca ggctgcccgc
3121 cggggatacc tcaccaagat cctgcatgtc ttccatggcc ttcttcctgg cttcctggtg
3181 aagatgagtg gcgacctgct ggagctggcc ttgaagttgc cccatgtcga ctacatcgag
3241 gaggactcct ctgtctttgc ccagagcatc ccgtggaacc tggagcggat tacccctcca
3301 cggtaccggg cggatgaata ccagcccccc gacggaggca gcctggtgga ggtgtatctc
3361 ctagacacca gcatacagag tgaccaccgg gaaatcgagg gcagggtcat ggtcaccgac
3421 ttcgagaatg tgcccgagga ggacgggacc cgcttccaca gacaggccag caagtgtgac
3481 agtcatggca cccacctggc aggggtggtc agcggccggg atgccggcgt ggccaagggt
3541 gccagcatgc gcagcctgcg cgtgctcaac tgccaaggga agggcacggt tagcggcacc
3601 ctcataggcc tggagtttat tcggaaaagc cagctggtcc agcctgtggg gccactggtg
3661 gtgctgctgc ccctggcggg tgggtacagc cgcgtcctca acgccgcctg ccagcgcctg
3721 gcgagggctg gggtcgtgct ggtcaccgct gccggcaact tccgggacga tgcctgcctc
3781 tactccccag cctcagctcc cgaggtcatc acagttgggg ccaccaatgc ccaagaccag
3841 ccggtgaccc tggggacttt ggggaccaac tttggccgct gtgtggacct ctttgcccca
3901 ggggaggaca tcattggtgc ctccagcgac tgcagcacct gctttgtgtc acagagtggg
3961 acatcacagg ctgctgccca cgtggctggc attgcagcca tgatgctgtc tgccgagccg
4021 gagctcaccc tggccgagtt gaggcagaga ctgatccact tctctgccaa agatgtcatc
4081 aatgaggcct ggttccctga ggaccagcgg gtactgaccc ccaacctggt ggccgccctg
4141 ccccccagca cccatggggc aggttggcag ctgttttgca ggactgtatg gtcagcacac
4201 tcggggccta cacggatggc cacagccgtc gcccgctgcg ccccagatga ggagctgctg
4261 agctgctcca gtttctccag gagtgggaag cggcggggcg agcgcatgga ggcccaaggg
4321 ggcaagctgg tctgccgggc ccacaacgct tttgggggtg agggtgtcta cgccattgcc
4381 aggtgctgcc tgctacccca ggccaactgc agcgtccaca cagctccacc agctgaggcc
4441 agcatgggga cccgtgtcca ctgccaccaa cagggccacg tcctcacagg ctgcagctcc
4501 cactgggagg tggaggacct tggcacccac aagccgcctg tgctgaggcc acgaggtcag
4561 cccaaccagt gcgtgggcca cagggaggcc agcatccacg cttcctgctg ccatgcccca
4621 ggtctggaat gcaaagtcaa ggagcatgga atcccggccc ctcaggagca ggtgaccgtg
4681 gcctgcgagg agggctggac cctgactggc tgcagtgccc tccctgggac ctcccacgtc
4741 ctgggggcct acgccgtaga caacacgtgt gtagtcagga gccgggacgt cagcactaca
4801 ggcagcacca gcgaaggggc cgtgacagcc gttgccatct gctgccggag ccggcacctg
4861 gcgcaggcct cccaggagct ccagtgatct agagcggccg cggatcccgc ccctctccct
4921 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
4981 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
5041 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
5101 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
5161 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
5221 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
5281 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
5341 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
5401 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
5461 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac
5521 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
5581 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
5641 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
5701 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
5761 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
5821 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
5881 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
5941 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
6001 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
6061 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctagacgcg
6121 tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
6181 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
6241 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
6301 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
6361 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
6421 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
6481 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
6541 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
6601 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
6661 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
6721 gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
6781 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
6841 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
6901 ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat
6961 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
7021 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
7081 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
7141 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
7201 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
7261 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
7321 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
7381 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
7441 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
7501 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
7561 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
7621 gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat
7681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
7741 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
7801 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
7861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
7921 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
7981 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
8041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
8101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
8161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
8221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
8281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
8341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
8401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
8461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
8521 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
8581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
8641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
8701 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
8761 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atcttatata
8821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
8881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
8941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
9001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
9061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
9121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
9181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
9241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
9301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
9361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
9421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
9481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
9541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
9601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
9661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
9721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
9781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
9841 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
9901 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
9961 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
10021 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
10081 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
10141 ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta caaacttagt
10201 agt
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