重组人软骨寡聚基质蛋白，HEK

产品英文名称：
Recombinant Mouse Complement Component C5a

英文简称:
mC5a

中文名称:
重组小鼠补体成分C5a

背景资料:
Mouse Complement 5a （C5a） is a glycoprotein which is a member of a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 77 a.a. peptide created by the C5a convertase proteolytic cleavage of C5 ? chain in the classical and alternative complement pathway （C4b2a3b， C3bBb3b）. The mouse C5a has four ? helices and thrC5a Mouse Recombinant produced in E.Coli is a single， non-glycosylated， Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa.The Mouse C5a is purified by proprietary chromatographic techniques. intrachain disulfide bonds which form a triple loop structure. C5a functions through G-protein coupled receptor （GPCR） （C5aR/CD88）. C5a is a e大肠杆菌中重组表达ective chemoattractant and anaphylatoxin which functions on all classes of leukocytes and on many other cell types including endothelial， smooth muscle， kidney， liver， and neural cells. Mouse C5a also mediates IL-8 release from bronchial epithelial cells. Furthermore， it triThe sequence of the first five N-terminal amino acids was determined and was found to be Asn-Leu-His-Leu-Leu.ers an oxidative surge in macrophages and neutrophils， causing the release of histamine in basophils and mast cells. The C5a anaphylatoxin activity on hepatocytes results indirectly from interaction with nonparenchymal cell via prostanoid secretion.

产品描述:
C5a Mouse Recombinant produced in E.Coli is a single， non-glycosylated， Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa.The Mouse C5a is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Leu-His-Leu-Leu.

溶解建议:
提供的重组小鼠补体成分C5a产品形式为冻干粉末.建议用无菌处理的18M-cm水溶解重组小鼠补体成分C5a，浓度不低于100ug/ml.后继实验过程中可根据实验要求进一步稀释.

保存建议：
重组小鼠补体成分C5a产品在室温保存3周，于-18°C以下长期保存.一旦溶解，于4°C保存2-7天.避免反复冻融
其他简介：

  ProSpec是一家细胞因子蛋白及相关抗体生产和研发品Pai。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种Z多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。