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Cotton Rat IL4 ELISA Kit (Custom ELISA)

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  • Cotton Rat IL4 ELISA Kit (Custom ELISA)Catalog
    Size
    Price
    LS-F409-1
    1 plate
    $875 USD
    Available Discounts
    10% off 2-4 units
    20% off 5+ units
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    SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F409 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Cotton Rat IL4. It is based upon a Custom assay principle.
    SpecificationsType
     
    Custom ELISA kit 

    All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.

    Target
     
    IL4
    Reactivity
     
    Cotton Rat
    Specificity
     
    The antibody pair provided in this kit recognizes cotton rat IL4.
    Manual
     

    Format
     
    96-Well Microplate
    Detection
     
    Colorimetric - 450nm (TMB)
    Measurement
     
    Quantitative
    Detection Range
     
    To be determined
    Sensitivity
     
    To be determined
    Storage
     
    Short term: 4°C; Long term: see manual.
    Kit Components
     
    Typical components include:
    • Microplate - Item A
    • Wash Buffer Concentrate (20x) - Item B
    • Standards - Item C
    • Assay Diluent A - Item D
    • Assay Diluent B - Item E
    • Biotinylated Detection Antibody
    • HRP-Streptavidin concentrate - Item G
    • TMB Substrate
    • Stop Solution - Item I
    • Adhesive Plate Sealers

    Protocol
     
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl standard or sample to each well.
    3. Incubate 2.5 hours at room temperature or overnight at 4°C.
    4. Add 100 µl prepared Detection Antibody to each well.
    5. Incubate 1 hour at room temperature.
    6. Add 100 µl prepared Streptavidin solution.
    7. Incubate 45 minutes at room temperature.
    8. Add 100 µl TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 minutes at room temperature.
    10. Add 50 µl Stop Solution to each well.
    11. Read at 450 nm immediately.

    Restrictions
     
    For research use only.
    Guarantee
     
    This elisa kit carries the LSBio Guarantee.
    About IL4
     
    P05112  NM_000589 NP_000580.1

     

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