Human GZMB / Granzyme B ELISA Kit (Sandwich ELISA) - LS-F32789 Catalog Size Price LS-F32789-1 1 plate $990 USD Request Bulk Add to Wish List Add to Cart SpecificationsPublicationsReviewsImagesProduct DescriptionLS-F32789 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human GZMB / Granzyme B in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of GZMB / Granzyme B as low as 9.375 picograms per milliliter.SpecificationsType
Store at 4°C. Stable for 6 months. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GzmBantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GzmB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GzmB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GzmB can be calculated. Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About GZMB / Granzyme B
P10144 NM_004131 NP_004122.2
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