Phospho-Threonine Antibody (P-Thr-Polyclonal) (Sepharose Bead)

品Pai:CST，产品名称：Phospho-Threonine Antibody (P-Thr-Polyclonal) (SepharoseBead Conjugate) #8781
英文名称：Phospho-Threonine Antibody (P-Thr-Polyclonal) (Sepharose® Bead Conjugate)
Product name：Phospho-Threonine Antibody (P-Thr-Polyclonal) (Sepharose^® Bead Conjugate) #8781
Isotype：Mouse IgG2a
Sensitivity：Transfected Only
Applications：IP ,
Purification： Polyclonal antibodies are produced by immunizing animals with synthetic phospho-Thr-containing peptides. Antibodies are purified by protein A and peptide affinity chromatography.
Background： Much of the dynamic behavior of cellular proteins, including the regulation of molecular interactions (1), subcellular localization (2), and transcriptional regulation (3) is controlled by a variety of post-translational modifications (4). Antibodies specific for these post-translational modifications are invaluable tools in the quest to understand normal and pathogenic molecular and cellular behavior.General protein modification antibodies are designed to react with modified amino acid residues (e.g. phospho-threonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context-independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs.

Specificity / Sensitivity:

Phospho-Threonine Antibody (P-Thr-Polyclonal) (Sepharose^® Bead Conjugate) immunoprecipitates proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. The antibody is phospho-specific and may cross-react with some phospho-serine-containing sequences. By ELISA, it recognizes a wide variety of threonine-phosphorylated peptides, and by 2D gel western blot analysis, it recognizes a large number of presumably threonine-phosphorylated proteins. CST recommends the use of Phospho-Threonine-Proline mAb (p-Thr-Pro-101) #9391 to detect proteins containing threonine followed by proline. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Species Reactivity:

Human, All Species Expected

Application	Dilutions
Immunoprecipitation	1:20

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.