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FITC标记的聚组氨酸标签抗体(C端)

产品信息
  • FITC标记的聚组氨酸标签抗体(C端)
    英文名称 Anti-His tag(CT)/FITC
    中文名称 FITC标记的聚组氨酸标签抗体(C端)
    别    名 HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag.  
    规格价格 100ul/2980元 购买        大包装/询价
    说 明 书 100ul  
    研究领域 染色质和核信号  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 fusion protein, HHHHHH tagged
    产品应用 IF=1:50-200  
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 Synthetic peptide: HHHHHH conjugated to KLH
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    产品介绍 background:
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

    标签抗体
    蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白

    1) 6xHis与不同的Sepharose纯化填料结合,可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
    2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
    3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: 细菌,杆状病毒和哺乳动物)
    4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌
    5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫。
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