Human FZD1 / Frizzled 1 ELISA Kit (Sandwich ELISA) - LS-F34051 Catalog Size Price LS-F34051-1 1 plate $1,090 USD Request Bulk Add to Wish List Add to CartPerformance Guaranteed SpecificationsPublicationsReviewsImagesPopular FZD1 / Frizzled 1 Elisa Kits Human FZD1 / Frizzled 1 ELISA Kit (Sandwich ELISA) - LS-F8285 Reactivity: Human Range: 0.156-10 ng/ml Human FZD1 / Frizzled 1 ELISA Kit (Sandwich ELISA) - LS-F11499 Reactivity: Human Range: 0.78-50 ng/ml Rat FZD1 / Frizzled 1 ELISA Kit (Custom ELISA) - LS-F16048 Reactivity: Rat Chicken FZD1 / Frizzled 1 ELISA Kit (Custom ELISA) - LS-F16049 Reactivity: Chicken Mouse FZD1 / Frizzled 1 ELISA Kit (Custom ELISA) - LS-F16050 Reactivity: MouseProduct DescriptionLS-F34051 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human FZD1 / Frizzled 1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of FZD1 / Frizzled 1 as low as 0.469 nanograms per millilter. More FZD1 / Frizzled 1 Products Top of PageSpecificationsType
Store at 4°C. Stable for 6 months. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FZD1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FZD1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FZD1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FZD1 can be calculated. Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About FZD1 / Frizzled 1
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. Q9UP38 NM_003505 NP_003496.1
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