磷酸化蛋白激酶C/p-PKCε抗体图片

公司专业供应的抗体，磷酸化蛋白激酶C/p-PKCε抗体图片是用于化学反应、分析化验、研究实验、教学实            验、化学配方使用的纯净化学品，产品品质，价格实惠，多种规格供应，售后完善。
英文名称  Anti-phospho-PKCEpsilon(Ser729)
中文名称 磷酸化蛋白激酶C/p-PKCε抗体图片
别    名 PKCepsilon(phosphoS729);PKCEpsilon(phosphoSer729);p-PKCEpsilon(Ser729);PKC-A;PKCalpha;AAG6;Agingassociatedgene6;PICK1;PKCA;PKCA;PKCA;PRKCA;ProteinKinaseCalpha;ProteinkinaseCalphatype;KPCE_HUMAN.

浓度1mg/1ml
规格0.1ml/100μg
抗体来源Rabbit
克隆类型polyclonal
交叉反应Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep
产品类型一抗磷酸化抗体
研究领域肿瘤免疫学信号转导细胞凋亡转录调节因子激酶和磷酸酶
蛋白分子量predictedmolecularweight：
84kDa 
性状LyophilizedorLiquid
免疫原KLHconjugatedsynthesisedphosphopeptidederivedfromhumanPKCEaroundthephosphorylationsiteofSer729
亚型IgG
纯化方法affinitypurifiedbyProteinA
储存液Preservative:15mMSodiumAzide,Constituents:1%BSA,0.01MPBS,pH7.4
产品应用WB=1:100-500ELISA=1:500-1000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500
（石蜡切片需做抗原修复）
notyettestedinotherapplications.
optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
保存条件Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
ImportantNoteThisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.
产品介绍ProteinkinaseC(PKC)isafamilyofserine-andthreonine-specificproteinkinasesthatcanbeactivatedbycalciumandthesecondmessengerdiacylglycerol.PKCfamilymembersphosphorylateawidevarietyofproteintargetsandareknowntobeinvolvedindiversecellularsignalingpathways.PKCfamilymembersalsoserveasmajorreceptorsforphorbolesters,aclassoftumorpromoters.EachmemberofthePKCfamilyhasaspecificexpressionprofileandisbelievedtoplayadistinctroleincells.TheproteinencodedbythisgeneisoneofthePKCfamilymembers.Thiskinasehasbeenshowntobeinvolvedinmanydifferentcellularfunctions,suchasneuronchannelactivation,apoptosis,cardioprotectionfromischemia,heatshockresponse,aswellasinsulinexocytosis.Knockoutstudiesinmicesuggestthatthiskinaseisimportantforlipopolysaccharide(LPS)-mediatedsignalinginactivatedmacrophagesandmayalsoplayaroleincontrollinganxiety-likebehavior.[providedbyRefSeq,Jul2008].
Function：
Calcium-independent,phospholipid-anddiacylglycerol(DAG)-dependentserine/threonine-proteinkinasethatplaysessentialrolesintheregulationofmultiplecellularprocesseslinkedtocytoskeletalproteins,suchascelladhesion,motility,migrationandcellcycle,functionsinneurongrowthandionchannelregulation,andisinvolvedinimmuneresponse,cancercellinvasionandregulationofapoptosis.Mediatescelladhesiontotheextracellularmatrixviaintegrin-dependentsignaling,bymediatingangiotensin-2-inducedactivationofintegrinbeta-1(ITGB1)incardiacfibroblasts.PhosphorylatesMARCKS,whichphosphorylatesandactivatesPTK2/FAK,leadingtothespreadofcardiomyocytes.InvolvedinthecontrolofthedirectionaltransportofITGB1inmesenchymalcellsbyphosphorylatingvimentin(VIM),anintermediatefilament(IF)protein.Inepithelialcells,associateswithandphosphorylateskeratin-8(KRT8),whichinducestargetingofdesmoplakinatdesmosomesandregulatescell-cellcontact.PhosphorylatesIQGAP1,whichbindstoCDC42,mediatingepithelialcell-celldetachmentpriortomigration.InHeLacells,contributestohepatocytegrowthfactor(HGF)-inducedcellmigration,andinhumancornealepithelialcells,playsacriticalroleinwoundhealingafteractivationbyHGF.Duringcytokinesis,formsacomplexwithYWHAB,whichiscrucialfordaughtercellseparation,andfacilitatesabscissionbyamechanismwhichmayimplicatetheregulationofRHOA.Incardiacmyocytes,regulatesmyofilamentfunctionandexcitationcouplingattheZ-lines,whereitisindirectlyassociatedwithF-actinviainteractionwithCOPB1.Duringendothelin-inducedcardiomyocytehypertrophy,mediatesactivationofPTK2/FAK,whichiscriticalforcardiomyocytesurvivalandregulationofsarcomerelength.PlaysaroleinthepathogenesisofdilatedcardiomyopathyviapersistentphosphorylationoftroponinI(TNNI3).Involvedinnervegrowthfactor(NFG)-inducedneuriteoutgrowthandneuronmorphologicalchangeindependentlyofitskinaseactivity,byinhibitionofRHOApathway,activationofCDC42andcytoskeletalrearrangement.Maybeinvolvedinpresynapticfacilitationbymediatingphorbolester-inducedsynapticpotentiation.Phosphorylatesgamma-aminobutyricacidreceptorsubunitgamma-2(GABRG2),whichreducestheresponseofGABAreceptorstoethanolandbenzodiazepinesandmaymediateacutetolerancetotheintoxicatingeffectsofethanol.UponPMAtreatment,phosphorylatesthecapsaicin-andheat-activatedcationchannelTRPV1,whichisrequiredforbradykinin-inducedsensitizationoftheheatresponseinnociceptiveneurons.IsabletoformacomplexwithPDLIM5andN-typecalciumchannel,andmayenhancechannelactivitiesandpotentiatesfastsynaptictransmissionbyphosphorylatingthepore-formingalphasubunitCACNA1B(CaV2.2).Inprostatecancercells,interactswithandphosphorylatesSTAT3,whichincreasesDNA-bindingandtranscriptionalactivityofSTAT3andseemstobeessentialforprostatecancercellinvasion.DownstreamofTLR4,playsanimportantroleinthelipopolysaccharide(LPS)-inducedimmuneresponsebyphosphorylatingandactivatingTICAM2/TRAM,whichinturnactivatesthetranscriptionfactorIRF3andsubsequentcytokinesproduction.Indifferentiatingerythroidprogenitors,isregulatedbyEPOandcontrolstheprotectionagainsttheTNFSF10/TRAIL-mediatedapoptosis,viaBCL2.Maybeinvolvedintheregulationoftheinsulin-inducedphosphorylationandactivationofAKT1. 
Subunit：
FormsaternarycomplexwithTRIM63andGN2BL1.CanformacomplexwithPDLIM5andN-typecalciumchannel.InteractswithCOPB1andYWHAB(Bysimilarity).InteractswithDGKQandSTAT3. 
SubcellularLocation：
Cytoplasm.Cytoplasm,cytoskeleton.Cellmembrane.Cytoplasm,perinuclearregion.Nucleus.Note=TranslocatedtoplasmamembraneinepithelialcellsstimulatedbyHGF.AssociatedwiththeGolgiattheperinuclearsiteinpre-passagefibroblasts.Inpassagingcells,translocatedtothecellperiphery.TranslocatedtothenucleusinPMA-treatedcells. 
Post-translationalmodifications：
hosphorylationonThr-566byPDPK1triggersautophosphorylationonSer-729.PhosphorylationinthehingedomainatSer-350byMAPK11orMAPK14,Ser-346byGSK3BandSer-368byautophosphorylationisrequiredforinteractionwithYWHAB. 
Similarity：
Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.PKCsubfamily. 
Contains1AGC-kinaseC-terminaldomain.
Contains1C2domain.
Contains2phorbol-ester/DAG-typezincfingers.
Contains1proteinkinasedomain.
Databaselinks:UniProtKB/Swiss-Prot:Q02156.1


磷酸化蛋白激酶C/p-PKCε抗体图片几种抗体概述：
1.抗角蛋白抗体（AKA），又称为抗丝集蛋白抗体或抗角质层抗体。AKA是类风湿关节炎早期诊断和判断预后的指标之一,其对RA患者的诊断的特异性为94% ,而其敏感性为47% ,并是一种鉴别RA和与多发性关节炎相关的丙型肝炎患者的有效检验标记物。
2.抗核周因子( APF)， 是定位于口腔黏附膜细胞胞质内的颗粒性蛋白复合物。可在RA患者的血清和关节液中测出，与性别、年龄无关。APF显示了较强的特异性（73%～99%）和敏感性。用间接免疫荧光法检测，方法精密度较差。
3.抗环瓜氨酸肽抗体（CCP），具有相对好的敏感度和特异性。其实，抗核周因子和抗角蛋白抗体，与CCP在化学结构上具有相关性，它们的表位都含有瓜氨酸，称为瓜氨酸相关自身免疫系统。
实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。

ELISAKitforHumanProteinCYR6178-5000pg/mL
人半胱氨酸蛋白酶YZ剂/胱抑素A(Cys-A)ELISA试剂盒3.9-250ng/mL
ELISAKitforHumanCystatin-A3.9-250ng/mL
人基质细胞衍生因子1(SDF-1)ELISA试剂盒0.78-50ng/mL
ELISAKitforHumanStromalcell-derivedfactor10.78-50ng/mL
人肌酸激酶同工酶MB(CK-MB)ELISA试剂盒6.25-400ng/mL
ELISAKitforHumanCreatinekinaseM-type6.25-400ng/mL
人衰变加速因子(DAF/CD55)ELISA试剂盒0.312-20ng/mL
ELISAKitforHumanComplementdecay-acceleratingfactor0.312-20ng/mL
人降钙素(CT)ELISA试剂盒7.8-500pg/mL
ELISAKitforHumanCalcitonin7.8-500pg/mL
人促黄体激素(LH)ELISA试剂盒0.156-10ng/mL
ELISAKitforHumanGlycoproteinhormonesalphachain0.156-10ng/mL
人绒毛膜促性腺激素β(β-CG)ELISA试剂盒62.5-4000pg/mL
ELISAKitforHumanChoriogonadotropinsubunitbeta62.5-4000pg/mL
人C肽(C-Peptide)ELISA试剂盒78-5000pg/mL
ELISAKitforHumanC-Peptide78-5000pg/mL
人组织蛋白酶B(CTS-B)ELISA试剂盒15.6-1000pg/mL
ELISAKitforHumanCathepsinB15.6-1000pg/mL
人过敏毒素/补体片断4a(C4a/C4b/C4d)ELISA试剂盒0.47-30nmol/L
磷酸化蛋白激酶C/p-PKCε抗体图片