免疫原： KLH conjugated synthetic peptide derived from human TSHR N-terminus
适应物种： 详见说明书
宿主： 详见说明书
标记物： 详见说明书
抗体名： 促甲状腺素受体抗体（N端）使用说明书
抗体英文名： Anti-TSHR (NT)
保质期： 详见说明书
抗原来源： Rabbit
克隆性： 多克隆
目录编号： 详见说明书
级别： 详见说明书
靶点： 详见说明书
数量： 大量
供应商： 上海博湖
规格： 0.2ml/200μg
英文名称 Anti-TSHR (NT) 中文名称促甲状腺素受体抗体（N端）使用说明书 别名 hTSHR I; hTSHRI; LGR 3; LGR3; MGC75129; Thyroid adenoma hyperfunctioning; Thyroid carcinoma with thyrotoxicosis; Thyroid Stimulating Hormone Receptor; Thyrotropin Receptor; Thyrotropin receptor I; TSH R; TSHR; TSHR_HUMAN. 浓度 1mg/1ml 规格 0.1ml/100μg 0.2ml/200μg 抗体来源 Rabbit 克隆类型 polyclonal 交叉反应 Human, Mouse, Rat, Dog, Cow, Sheep 产品类型一抗 研究领域免疫学神经生物学信号转导生长因子和激素细胞膜受体 蛋白分子量 predicted molecular weight: 86kDa 性状 Lyophilized or Liquid 免疫原 KLH conjugated synthetic peptide derived from human TSHR N-terminus 亚型 IgG 纯化方法 affinity purified by Protein A 储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 促甲状腺素受体抗体（N端）使用说明书产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 产品介绍 The glycoprotein hormone receptor family consists of the luteinizing hormone receptor, the follicle-stimulating hormone receptor, and the thyroid stimulating hormone(TSH) receptor. TSH, which is released from the pituitary gland, binds to the TSH receptor on thyroid cells to control size and function of the thyroid gland (De Felice et al. 2004). The TSH receptor signals through Gs to elevate intracellular cAMP in the thyroid gland, which regulates iodide uptake, and transcription of thyroglobulin (Tg), thyroid peroxidase (TPO), and sodium-iodide symporter. The TSH receptor also signals Gq and phospholipase C to regulat iodide efflux, H2O2 production, and thyroglobulin iodination. Autoimmunity to the TSH receptor causes hyperthyroidism (Graves disease) or hypothyroidism (Hashimoto thyroiditis) when the autoantibodies function as agonists or antagonists, respectively, at the TSH receptor (Rapoport and McLachlan, 2001; Davies et al., 2002). Millipore’s cloned human TSH receptor-expressing cell line is made in the Chem-10 host, which supports high levels of recombinant TSH receptor expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between TSH and its ligands. Function : Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). Subunit : Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function. Subcellular Location : Cell membrane; Multi-pass membrane protein. Tissue Specificity : Expressed in the thyroid. DISEASE : Note=Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers). Similarity : Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. Contains 7 LRR (leucine-rich) repeats. Database links : UniProtKB/Swiss-Prot: P16473.2 TSHR是垂体前叶嗜碱细胞分泌的一种糖蛋白，直接作用于甲状腺，并能影响其结构功能。该抗体主要用于垂体肿瘤功能性分类的研究。
