磷酸化-丝裂原活化蛋白激酶p38抗体（P-p38 MAPK）图片

磷酸化-丝裂原活化蛋白激酶p38抗体（P-p38 MAPK）图片是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品，产品品质，价格实惠，多种规格供应，售后完善。
英文名称  Anti-Phospho-P38 MAPK (Thr180/Tyr182)
中文名称  磷酸化-丝裂原活化蛋白激酶p38抗体（P-p38 MAPK）图片
别    名  P38 MAPK(Phospho-Thr180); phospho-MAPK14(Thr180/Tyr182); MAPK14(phospho Thr180/Tyr182); CSAID Binding Protein 1; CSAID binding protein; CSAID-binding protein; Csaids binding protein; CSBP 1; CSBP 2; CSBP; CSBP1; CSBP2; CSPB 1; CSPB1; Cytokine suppressive

浓    度  1mg/1ml
规 格  0.1ml/100μg
抗体来源  Rabbit
克隆类型  polyclonal
交叉反应  Human, Mouse, Rat, Dog, Rabbit
产品类型  一抗 磷酸化抗体  
研究领域  肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
蛋白分子量  predicted molecular weight: 42kDa 
性    状  Lyophilized or Liquid
免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182
亚    型  IgG
纯化方法   affinity purified by Protein A
储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide


磷酸化-丝裂原活化蛋白激酶p38抗体（P-p38 MAPK）图片产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases(MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Function : Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD4 mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at 'Thr-113'.
Subunit : Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 and GADD4. Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, FAM48A and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B.
Subcellular Location : Cytoplasm. Nucleus.
Post-translational modifications : Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which a ctivates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16.
Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3.
Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.
Similarity : Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Database links :
UniProtKB/Swiss-Prot: Q16539.3
Entrez Gene: 403856 Dog
Entrez Gene: 1432 Human
Entrez Gene: 26416 Mouse
Entrez Gene: 81649 Rat
GenBank: NM_001315 Human
GenBank: NM_139012 Human
GenBank: NM_011951 Mouse
GenBank: NM_031020 Rat
激酶和磷酸酶（Kinases and Phosphatases）
丝裂原活化蛋白激酶p38(p38 MAPK、磷酸化pERK)参与细胞生长、增殖、分化、死亡及细胞间的功能同步等多种生理过程.
P-p38MAPK是丝裂原活化蛋白激酶家族中的成员之一，大量研究显示p38在能量代谢中具有广泛的作用。p38参与脂肪组织、骨骼肌、胰岛细胞和肝脏等组织、器官的能量代谢.分子量：38KDa
p38 MAPK：作为细胞信号传递系统的交汇点，细胞内普遍存在的一条信号转导通路。细胞外的物理应激因子，如高渗透压、热休克、紫外线以及细胞因子、内毒素脂多糖（LPS）等都能激活该途径，诱导细胞内蛋白质合成与分泌、细胞分化及凋亡等生物效应。p38 MAPK还能与细胞内其他信号通路之间相互作用，是细胞内信号传递系统的交汇点或共同通路。p38 MAPK一旦被激活后，可以使一些转录因子如CREB、转录活化因子-1（activating factor-1， ATF-1）、ATF-2及活化蛋白-1(AP-1)等的丝氨酸和苏氨酸位点磷酸化，活化这些转录因子，从而调节目的基因的表达。 p38（丝氨酸位点）磷酸化后可以直接激活转录因子，参与机体的应激反应。


磷酸化-丝裂原活化蛋白激酶p38抗体（P-p38 MAPK）图片具有全、新、优、品、好四大特点：
全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。
新：产品更新速度较快，基本上每周都有新产品出现。
优：产品质量好，投诉比较少。
好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
我们提供的标记服务，可根据您科研的需求，满足您的需要，质量保证，价格合理。
一、标记流程
委托标记服务，请下载并详细填写《委托标记申请单》，待我公司标记室技术人员研究、确认后答复，并办理委托标记事宜。
二、各种标记物制备的收费标准（RMB） 
我们不但提供抗体和大分子蛋白的标记服务，还提供小分子多肽及小分子蛋白的各种标记服务，提供标记化合物的服务（此化合物必须适用于标记），价格另议。
一抗和二坑的区别：
抗体就是平常所说的抗体，即能和抗原特异性结合。
第二抗体是能和抗体结合的，即抗体的抗体。主要用于检测抗体的存在。
一抗是针对抗原的抗体，二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。也就是说，抗原进入机体刺激机体免疫系统产生免疫应答，由B细胞可以产生与相应抗原发生特异性结合的特殊蛋白质。
一抗二抗都是一种可以特异结合别的物质的基团，而且一抗可以至少结合两种其他基团（底物和二抗）。
一抗：可以特异结合底物，就是识别出我们想要检测的东西。一抗和底物结合与否用肉眼是看不出来的。
二抗：可以和一抗结合，并带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团），作用是检测一抗。 如果一抗自己带有可以被检测出的标记(如带荧光、放射性、化学发光或显色基团），则不需要二抗。但这样成本很高，因为一种一抗只识别一种底物。所以如今的设计一般是二抗带上可检测标记，再来检测一抗。而一抗识别底物。这样，当一抗结合到底物上，就可以通过二抗检测出来。 

Western二抗稀释液  进口/国产
组织蛋白抽提试剂  进口/国产
考马斯亮蓝R250  进口/国产
ELISA终止液  进口/国产
AEC显色试剂盒（20×）  进口/国产
酸敏感离子通道蛋白3抗体  Anti-ASIC3  WB IHC-P IHC-F IF  100ul
天门冬酸β羟化酶抗体  Anti-Aspartate beta hydroxylase  WB IHC-P IHC-F IF  100ul
细胞骨架肌动蛋白样蛋白3抗体  Anti-ARP3  WB IHC-P IHC-F IF  100ul
膜粘连蛋白8抗体  Anti-Annexin A8  WB IHC-P IHC-F IF  100ul
水通道蛋白8抗体  Anti-Aquaporin 8  WB IHC-P IHC-F IF  100ul
19号染色体开放阅读框28抗体  Anti-C19orf28  WB IHC-P IHC-F IF  100ul
肌红蛋白抗体  Anti-Myoglobin  WB IHC-P IHC-F IF  100ul
错配修复蛋白6抗体  Anti-MSH6  WB IHC-P IHC-F IF  100ul
乳腺珠蛋白2抗体  Anti-Mammaglobin B  WB IHC-P IHC-F IF  100ul
减数分裂缺陷蛋白1抗体  Anti-MEI-1  WB IHC-P IHC-F IF  100ul
ACHN(人肾癌细胞) 5×106cells/瓶×2
CL-0173NRK(大鼠肾细胞)5×106cells/瓶×2
PDGFRA Others Rat 大鼠 PDGFRa / CD1a 人细胞裂解液 (阳性对照)
HepG2细胞，肝细胞癌 人T淋巴瘤细胞系,Hut-102细胞 小鼠胚胎成纤维细胞;NIH/3T3
牛肾细胞;MDBK(NBL-1)
HA Others H5N1 甲型流感 H5N1 (A/VietNam/1203/2004) 血凝素 (Hemagglutinin / HA) 人细胞裂解液
人肺微血管内皮细胞HPMEC
CASK Others Human 人 CASK Kinase 杆状病毒-昆虫细胞裂解液 (阳性对照)
猪肾细胞;PK(15)
BRL 3A细胞，大鼠肝细胞 人转移癌细胞,AsPc-1细胞 CM-R109大鼠脑成纤维细胞完全培养基100mL
大鼠肾上腺嗜铬细胞瘤细胞(低分化);PC-12(低分化)
IL20RA Others Human 人 IL20Rα 人细胞裂解液 (阳性对照)
磷酸化-丝裂原活化蛋白激酶p38抗体（P-p38 MAPK）图片ACHN(人肾癌细胞) 5×106cells/瓶×2
CL-0173NRK(大鼠肾细胞)5×106cells/瓶×2
PDGFRA Others Rat 大鼠 PDGFRa / CD1a 人细胞裂解液 (阳性对照)
HepG2细胞，肝细胞癌 人T淋巴瘤细胞系,Hut-102细胞 小鼠胚胎成纤维细胞;NIH/3T3
牛肾细胞;MDBK(NBL-1)
HA Others H5N1 甲型流感 H5N1 (A/VietNam/1203/2004) 血凝素 (Hemagglutinin / HA) 人细胞裂解液